Synergistic interaction and the role of C-terminus in the activation of TRAAK K+ channels by pressure, free fatty acids and alkali
DC Field | Value | Language |
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dc.contributor.author | Kim, Yangmi | - |
dc.contributor.author | Bang, Hyoweon | - |
dc.contributor.author | Gnatenco, Carmen | - |
dc.contributor.author | Kim, Donghee | - |
dc.date.available | 2019-06-26T01:28:09Z | - |
dc.date.issued | 2001-02 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.issn | 1432-2013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/26309 | - |
dc.description.abstract | TRAAK is a member of the tandem-pore K+ channel family, and is expressed mainly in the brain. Using rat TRAAK (rTRAAK), we studied its single-channel kinetics, interactive modes of activation, and the role of the C-terminus on its pressure-, fatty-acid- and pH-sensitivity. When expressed in COS-7 cells, rTRAAK showed a mildly inwardly rectifying single-channel current/voltage relationship in symmetrical 140 mM KCl. Unlike TREK-1 and TREK-2, which are activated by acidic conditions, rTRAAK was activated by alkali conditions, such that a change in intracellular pH from 7.3 to 8.3 and 8.8 increased channel activity 5- and 14-fold, respectively. Pressure and alkali produced a strong synergistic activation, and pressure and arachidonic acid (AA) produced a mild synergistic activation. The only additive effect was observed with alkali and AA. Replacing the C-terminus of rTRAAK with that of TASK-1 or TASK-3 did not affect the response to pressure, AA or alkali. In contrast, replacing the C-terminus of TREK-2 with that of TASK-3 abolished the sensitivity to AA and acid, but not to pressure. These results show that rTRAAK is an alkali-sensing K+ channel that shows synergistic activation with pressure, and that the mechanism of activation of rTRAAK and TREK by free fatty acids are different. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Synergistic interaction and the role of C-terminus in the activation of TRAAK K+ channels by pressure, free fatty acids and alkali | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s004240000496 | - |
dc.identifier.bibliographicCitation | Pflugers Archiv European Journal of Physiology, v.442, no.1, pp 64 - 72 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000168398000009 | - |
dc.identifier.scopusid | 2-s2.0-0035054301 | - |
dc.citation.endPage | 72 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 64 | - |
dc.citation.title | Pflugers Archiv European Journal of Physiology | - |
dc.citation.volume | 442 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | TRAAK K+ channels | - |
dc.subject.keywordAuthor | Mechanosensitivity | - |
dc.subject.keywordAuthor | Synergism | - |
dc.subject.keywordAuthor | Pressure | - |
dc.subject.keywordAuthor | Free fatty acids | - |
dc.subject.keywordAuthor | Alkali | - |
dc.subject.keywordPlus | 2 PORE DOMAINS | - |
dc.subject.keywordPlus | ARACHIDONIC-ACID | - |
dc.subject.keywordPlus | POTASSIUM CHANNEL | - |
dc.subject.keywordPlus | FUNCTIONAL EXPRESSION | - |
dc.subject.keywordPlus | OUTWARD RECTIFIER | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | TASK-1 | - |
dc.subject.keywordPlus | TREK-1 | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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