Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis
DC Field | Value | Language |
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dc.contributor.author | Park, Jong Pil | - |
dc.contributor.author | Lee, Seok Jae | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Lee, Kyung-Bok | - |
dc.contributor.author | Choi, Insung S. | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.contributor.author | Kim, Min-Gon | - |
dc.contributor.author | Chung, Bong Hyun | - |
dc.date.available | 2019-07-08T08:55:12Z | - |
dc.date.issued | 2004-04 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27636 | - |
dc.description.abstract | In this study, a simple procedure is described for patterning biotin on a glass substrate and then selectively immobilizing proteins of interest onto the biotin-patterned surface. Microcontact printing (CP) was used to generate the micropattern of biotin and to demonstrate the selective immobilization of proteins by using enhanced green fluorescent protein (EGFP) as a model protein, of which the C-terminus was fused to a core streptavidin (cSA) gene of Streptomyces avidinii. Confocal fluorescence microscopy was used to visualize the pattern of the immobilized protein (EGFP-cSA), and surface plasmon resonance was used to characterize biological activity of the immobilized EGFP-cSA. The results suggest that this strategy, which consists of a combination of muCP and cSA-fused proteins, is an effective way for fabricating biologically active substrates that are suitable for a wide variety of applications, one such being the use in protein-protein assays. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/BF02932997 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.9, no.2, pp 137 - 142 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000221239100011 | - |
dc.identifier.scopusid | 2-s2.0-4644248503 | - |
dc.citation.endPage | 142 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 137 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | microcontact printing (mu CP) | - |
dc.subject.keywordAuthor | pattern generation | - |
dc.subject.keywordAuthor | protein-protein assay | - |
dc.subject.keywordAuthor | surface plasmon resonance | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | MONOLAYERS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | FILMS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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