Directed self-assembly of gold binding polypeptide-protein A fusion proteins for development of gold nanoparticle-based SPR immunosensors
DC Field | Value | Language |
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dc.contributor.author | Ko, Sungho | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Kim, Hyo-Sop | - |
dc.contributor.author | kim, Jae-Ho | - |
dc.contributor.author | Cho, Yong-Jin | - |
dc.date.available | 2019-07-11T05:55:14Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27827 | - |
dc.description.abstract | Effective immobilization of antibodies on a sensing platform and sensitivity enhancement are crucial in designing surface plasmon resonance (SPR) immunosensors. Colloidal gold nanoparticles (AuNPs) were directly assembled onto a surface of SPR Au chip via 2-aminoethanethiol for the enhancement of sensitivity as a label-free detection system. SEM image showed most AuNPs were uniformly distributed over the surface. A novel fusion protein was constructed by genetically fusing gold binding polypeptides (GBP) to protein A (ProA) as a crosslinker for effective immobilization of antibodies. The resulting GBP-ProA protein was directly self-immobilized onto both bare and AuNPs-assembled SPR chip surfaces via the GBP portion, followed by the oriented binding of human immunoglobulin G (hlgG) onto the ProA domain targeting the Fc region of antibodies and anti-hlgG in series. Furthermore, anti-Salmonella antibodies were immobilized onto both GBP-ProA layered chips for detection of Salmonella typhimurium. SPIR analyses indicated the signal increases for successive binding of hIgG and anti-hIgG onto the GBP-ProA layered AuNPs-assembled chip were higher (about 92 and 30%, respectively) than that onto the identically treated bare chip. This signal enhancement in the AuNPs-assembled chip also caused a 10-fold increased sensitivity in detection of S. typhimurium compared to the bare one. These results demonstrate the direct assembly of AuNPs onto a SPR chip could enhance the signal in biomolecular interaction events, and the GBP-ProA protein could be a valuable crosslinker for simple and oriented immobilization of antibodies onto Au chip surfaces without any surface chemical modification. (c) 2009 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Directed self-assembly of gold binding polypeptide-protein A fusion proteins for development of gold nanoparticle-based SPR immunosensors | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2009.01.030 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.24, no.8, pp 2592 - 2597 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000265811000046 | - |
dc.identifier.scopusid | 2-s2.0-62949153548 | - |
dc.citation.endPage | 2597 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2592 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 24 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Surface plasmon resonance | - |
dc.subject.keywordAuthor | Immunosensor | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Fusion protein | - |
dc.subject.keywordAuthor | Gold binding polypeptides | - |
dc.subject.keywordAuthor | Protein A | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | CHIP | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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