양배추의 미생물 오염도 평가 및 제어

Abstract

In this study, untreated (UT), water soaking (WT), and sanitizing solutions [chlorine at 100 ppm (CL); ethanol at 10% (ET); hydrogen peroxide at 1% (HP); chlorine at 100 ppm + ethanol at 10%(CE); chlorine at 100 ppm + hydrogen peroxide at 1% (CH); ethanol at 10%+ hydrogen peroxide at 1% (EH); chlorine at 100 ppm + ethanol at 10%+ hydrogen peroxide at 1% (CEH)] were compared in terms of their antimicrobial effectiveness against natural microflora of wild cabbage (Brassica oleracea var. capitata). All samples were kept in sanitizing solutions for 2 min, and effectiveness of sanitizing agents was evaluated based on number of decimal reduction of total aerobic mesophilic, total coliforms, E. coli, lactic acid bacteria, and yeast and mold counts. Average initial levels of these organisms in samples were 9.21 ± 0.15, 6.60 ± 0.06, 6.08 ± 0.03, and 3.66 ± 0.08 log10 CFU/g for total aerobic mesophilic bacteria, total coliforms, lactic acid bacteria, and yeasts and molds, respectively. Escherichia coli was not detected in any tested samples. Decimal reduction of populations of total aerobic mesophilic, total coliforms, E. coli, lactic acid bacteria, and yeasts and molds were: in WT 8.09, 5.36, 5.82, and 3.57 log10 CFU/ g; in CL 7.39, 4.10, 5.24, 2.45 log10 CFU/g; in ET 6.78, 4.23, 5.20, 2.50 log10 CFU/g; in HP 6.11, 4.27, 5.28, 2.46 log10 CFU/g; in CE 6.18, 4.26, 5.31, 2.49 log10 CFU/g; in CH 6.10, 3.77, 5.33, 2.46 log10 CFU/g; in EH 6.07, 3.82, 4.76, 2.41 log10 CFU/g; and in CEH 5.27, 3.45, 4.45, 2.15 log10 CFU/g, respectively. Statistical analysis of the results showed effectiveness of CEH sanitizing solution for elimination of microbial contamination was the highest among all sanitizer treatments.