하수오 지상부의 활성 성분

Abstract

aging in Korean folk medicine. Specially aerial parts has been used for insomnia and sedative agent. In order to investigatethe efficacy of antioxidant activity on these aerial parts, the bio-activity guided fraction and isolation of physiologically activesubstance were performed. H2O, 30%, 60%, 100% MeOH were examined antioxidant activity by DPPH method. It wasrevealed that 30%, 60% MeOH fractions have significantly antioxidant activity. From 60% MeOH fraction, six known fla-vonoid glycosides (quercetin, quercetin 3-O-β-D-galactopyranosyl-4'-O-glucopyranoside-3'-methylether, quercetin 3-O-α-L-rhamnopyranoside, quercetin 3-O-α-L-rhamnopyrano side-4'-methylether, quercetin 3-O-β-D-galactopyranoside, quercetin 3-O-β-D-glucopyranoside) and 3,4-dihydrocinnamic acid were isolated. To investigate the antioxidant activities of each com-pounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipo-protein (LDL) with TBARS asay and anti-acetylcholinestrase activity with Ellman's Test. Four compounds (II, IV, VI, VII)of quercetin glycosides showed significant activity.Keywords . Polygonum multiflorum, DPPH method, TBARS assay, Ellman's Test, quercetin glycosides... (Polygonum multiflorum). .... (Polygonaceae).