쥐 해마의 발달과정에서 N-methyl-D-aspartate 신경독성과 N-methyl-D-aspartate 수용체 2B 서브유닛의 발현양상
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤영철 | - |
dc.contributor.author | 박태환 | - |
dc.contributor.author | 권오상 | - |
dc.contributor.author | 채수안 | - |
dc.contributor.author | 이정수 | - |
dc.date.available | 2019-07-24T05:58:24Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1225-7044 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/30453 | - |
dc.description.abstract | Background: The developing brain has a distinctive set of characteristics that make it have different susceptibility to excitotoxins. Using primary tissue cultures of rat hippocampus, we investigated the developmental susceptibility to N-methyl-D-aspartic acid (NMDA)-induced cell death at various days in vitro in relation to the appearance of Bcl-2 protein and NMDA receptor 2B subunit. Methods: Six, 12, and 18 days-in-vitro (DIV) hippocampal tissue cultures derived from 7-day-old Sprague-Dawley rat pups were used. Each group was treated with 100 μM NMDA in 5% CO2 incubator at 36℃ for 30 min. A western blot was then performed for the NeuN, Bcl-2 and NMDA receptor 2B subunit and propidium Iodide (PI) staining. Results: The NeuN and Bcl-2 were most highly expressed in 12 DIV tissues. The reductions of the NeuN and Bcl-2 protein expressions by NMDA were significant at the 12 and the 18 DIV tissues, but less at 6 DIV tissues (p<0.05). The PI staining showed that the area of fluorescence of the 7 DIV tissues after NMDA exposure was less than the DIV 13 and 19 tissues. Without NMDA treatment, the NMDA receptor 2B subunit protein expressions at the 6 DIV tissues were highest and decreased with maturation. Conclusions: These results suggest that the immature tissues were more resistant to NMDA toxicity than the mature tissues, and further studies are needed to establish its relationship with the Bcl-2 protein and NMDA receptor 2B subunit. | - |
dc.format.extent | 8 | - |
dc.publisher | 대한신경과학회 | - |
dc.title | 쥐 해마의 발달과정에서 N-methyl-D-aspartate 신경독성과 N-methyl-D-aspartate 수용체 2B 서브유닛의 발현양상 | - |
dc.title.alternative | Developmental Differences in NMDA Neurototoxicity and the Expression of NMDA Receptor 2B Subunit of Rat Hippocampus | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 대한신경과학회지, v.25, no.3, pp 364 - 371 | - |
dc.identifier.kciid | ART001194069 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 371 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 364 | - |
dc.citation.title | 대한신경과학회지 | - |
dc.citation.volume | 25 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Hippocampus | - |
dc.subject.keywordAuthor | Bcl2 | - |
dc.subject.keywordAuthor | NeuN protein | - |
dc.subject.keywordAuthor | N-methyl-D-aspartate | - |
dc.subject.keywordAuthor | NR2B protein | - |
dc.description.journalRegisteredClass | kci | - |
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