한국산 곡류의 Beauvericin의 오염도 조사 및 Beauvericin과 Enniatin 유도체 생성조건

Abstract

The productions of beauvericin and enniatins H, I, and MK1688 by Fusarium oxysporum KFCC 11363P were investigated on rice substrate at four temperatures (15, 20, 25, and 30oC) and three moisture contents (10, 20, and 40%). The largest amount of beauvericin (718.0 μg/g) was produced at 25oC, and maximum levels of enniatin H (781.9 μg/g), I (725.8 μg/g), and MK1688 (425.8 μg/g) were measured by high pressure liquid chromatography (HPLC) at the same temperature. The optimal moisture content for the production of beauvericin and enniatins H, I, and MK1688 was 40%, and the trace amounts of these toxins were observed at 10% moisture content. Sixty five grain samples (n=65) were tested for the monitoring of beauvericin. This mycotoxin was detected in six grain samples including three maize, two barley, and one wheat samples. The highest contamination level of beauvericin was observed in maize sample (0.23 μg/g).