사람 눈에서 Transglutaminase 4의 발현과 분포양상Immunohistochemical Localization of Transglutaminase 4 in the Human Eye
- Authors
- Chun, Yeoun Sook; Seo, Seung Hee; Kim, In Gyu; Wee, Won Ryang; Lee, Jin Hak
- Issue Date
- 2003
- Publisher
- 대한안과학회
- Keywords
- Immunohistochemial staining; Ocular tissues; Transglutaminase 4
- Citation
- 대한안과학회지, v.44, no.8, pp 22 - 1878
- Pages
- 1857
- Journal Title
- 대한안과학회지
- Volume
- 44
- Number
- 8
- Start Page
- 22
- End Page
- 1878
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32097
- ISSN
- 0378-6471
2092-9374
- Abstract
- 목적 : Transglutaminase 4 (TGase 4)는 TGase효소군에 속하는 분비성 효소로서 단백질의 전사 후 수식에 관여한다. 사람 눈에서 이 효소의 기능을 규명하기 위하여 이의 발현과 분포양상을 알아보고자 하였다.
대상과 방법 : 사후의 정상 사람 눈(태생 40+1 주, 생후 2개월, 48, 66, 76세)에서 사람 TGase 4에 대한 단세포군 항체 (anti-human TGase 4 monoclonal antibody)를 이용하여 간접면역조직화학 염색을 시행하였다.
결과 : 눈물샘, 각막상피세포와 내피세포, 윤부 상피세포, 결막 상피세포, 수정체 상피세포, 망막(속분절 시세포층, 외경계막, 외망상층, 내망상층, 망막신경섬유층, 내경계막), 동공산대근과 동공괄약근, 모양체근, 모양체 무색소상피, 섬유주, 혈관내피세포에서 뚜렷이 발견되었다. 결막 간질, 맥락막, 전테논낭에서는 매우 약하게 발견되었다. 각막간질, 수정체핵, 홍채간질, 모양체돌기, 공막, 외안근, 시신경에서는 발견되지 않았다.
결론 : 사람 눈에서 TGase 4는 다른 TGase isoform들과 다른 분포양상을 보였다. 이 결과는 향후 눈에서 TGase 4의 기능을 규명하는데 도움을 줄 것으로 기대된다.
Purpose: Transglutaminase 4 (TGase 4) belongs to a family of enzymes that catalyzes the post-translational modification of proteins. In an attempt to establish its physiological function(s), the distribution of TGase 4 expression in the human eye was determined.
Methods: Ocular tissues obtained from five human whole eyeball postmortem (40+1 weeks at gestation age, 2 months, 48, 66, 76 years) were stained with monoclonal antibodies against human TGase 4 using indirect immunohistochemical method.
Results: TGase 4 was found in the lacrimal glands, corneal epithelium and endothelium, conjunctival epithelium, lens epithelium, retina (inner segment of photoreceptor, external limiting membrane, outer plexiform layer, inner plexiform layer, retinal nerve fiber layer and internal limiting membrane), iris, ciliary muscle, ciliary nonpigmented epithelium and trabecular meshwork. Endothelium of blood vessels in all ocular tissues was also stained. Conjunctival stroma, choroid, anterior tenon’s capsule were faintly stained. No evidence of immunostaining for TGase 4 was found in the corneal stroma, iris stroma, lens nucleus, ciliary process, sclera, extraocular muscle and optic nerve.
Conclusions: The expression pattern of TGase 4 was different from those of other TGase isoforms in the human eye. This result may be helpful in further investigation of the role of TGase 4 in the ocular tissue.
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