Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity
DC Field | Value | Language |
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dc.contributor.author | Hong, Yeojin | - |
dc.contributor.author | Truong, Anh Duc | - |
dc.contributor.author | Lee, Janggeun | - |
dc.contributor.author | Lee, Kyungbaek | - |
dc.contributor.author | Kim, Geun-Bae | - |
dc.contributor.author | Heo, Kang-Nyeong | - |
dc.contributor.author | Lillehoj, Hyun S. | - |
dc.contributor.author | Hong, Yeong Ho | - |
dc.date.available | 2019-08-09T07:56:39Z | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 1011-2367 | - |
dc.identifier.issn | 1976-5517 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32697 | - |
dc.description.abstract | Objective: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. Methods: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. Results: The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. Conclusion: Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC | - |
dc.title | Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity | - |
dc.type | Article | - |
dc.identifier.doi | 10.5713/ajas.18.0571 | - |
dc.identifier.bibliographicCitation | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.32, no.7, pp 1052 - 1061 | - |
dc.identifier.kciid | ART002480020 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000472147700016 | - |
dc.identifier.scopusid | 2-s2.0-85072089912 | - |
dc.citation.endPage | 1061 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1052 | - |
dc.citation.title | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | - |
dc.citation.volume | 32 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Antimicrobial Peptides | - |
dc.subject.keywordAuthor | Liver-expressed Antimicrobial Peptide 2 (LEAP-2) | - |
dc.subject.keywordAuthor | Duck | - |
dc.subject.keywordAuthor | Disulfide Bond | - |
dc.subject.keywordAuthor | Pathogens | - |
dc.subject.keywordPlus | DISULFIDE BONDS | - |
dc.subject.keywordPlus | LEAP-2 | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | GENES | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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