Broad-Spectrum Gene Repression Using Scaffold Engineering of Synthetic sRNAs
DC Field | Value | Language |
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dc.contributor.author | Noh, Minho | - |
dc.contributor.author | Yoo, Seung Min | - |
dc.contributor.author | Yang, Dongsoo | - |
dc.contributor.author | Lee, Sang Yup | - |
dc.date.available | 2019-08-09T07:58:44Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 2161-5063 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32748 | - |
dc.description.abstract | Gene expression regulation in broad-spectrum range is critical for constructing cell factories and genetic circuits to balance and control system-wide fluxes. Synthetic small regulatory RNAs (sRNAs) effectively regulate gene expression at the translational level by modulating an mRNA-binding chance and sRNA abundance; however, it can control target gene expression only within the limit of the intrinsic repression ability of sRNAs. Here, we systematically mutated a SgrS scaffold as a model sRNA by dividing the Hfq-binding module of the sRNA into the three regions: the A/U-rich sequence, the stem, and the hairpin loop, and examined how efficiently the mutants suppressed DsRed2 expression. By doing this, we found that a scaffold with an altered A/U-rich sequence (CUUU) and stem length and that with altered A/U-rich sequence (GCAC) showed a 3-fold stronger and a 3-fold weaker repression than the original scaffold, respectively. For practical application of altered scaffolds, proof-of-concept experiments were performed by constructing a library of 67 synthetic sRNAs with the strongest scaffold, each one targeting a different rationally selected gene, and using this library to enhance cadaverine production in Escherichia coli, yielding in 27% increase (1.67 g/L in flask cultivation, 13.7 g/L in fed-batch cultivation). Synthetic sRNAs with engineered sRNA scaffolds could be useful in modulating gene expression for strain improvement. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Broad-Spectrum Gene Repression Using Scaffold Engineering of Synthetic sRNAs | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acssynbio.9b00165 | - |
dc.identifier.bibliographicCitation | ACS SYNTHETIC BIOLOGY, v.8, no.6, pp 1452 - 1461 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000473115700024 | - |
dc.identifier.scopusid | 2-s2.0-85067681973 | - |
dc.citation.endPage | 1461 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1452 | - |
dc.citation.title | ACS SYNTHETIC BIOLOGY | - |
dc.citation.volume | 8 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | gene repression | - |
dc.subject.keywordAuthor | Hfq-binding region | - |
dc.subject.keywordAuthor | knockdown | - |
dc.subject.keywordAuthor | synthetic sRNA | - |
dc.subject.keywordPlus | BACTERIAL SMALL RNA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | REGULATORY RNAS | - |
dc.subject.keywordPlus | HFQ | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DESIGN | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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