Combination treatment with auranofin and nutlin-3a induces synergistic cytotoxicity in breast cancer cells
DC Field | Value | Language |
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dc.contributor.author | Ye, Dong-Jin | - |
dc.contributor.author | Kwon, Yeo-Jung | - |
dc.contributor.author | Baek, Hyoung-Seok | - |
dc.contributor.author | Cho, Eunah | - |
dc.contributor.author | Kwon, Tae-Uk | - |
dc.contributor.author | Chun, Young-Jin | - |
dc.date.available | 2019-08-13T04:57:01Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 1528-7394 | - |
dc.identifier.issn | 1087-2620 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32828 | - |
dc.description.abstract | Auranofin is a gold complex categorized as an anti-rheumatic agent. Recently, several investigators suggested that auranofin may act as a potent anti-cancer drug for breast tumors. Nutlin-3a is a cis-imidazoline analog which prevents interaction between mouse double minute 2 homolog (MDM2) and the tumor suppressor p53. The aim of this study was to examine cell growth inhibition mediated by auranofin or nutlin-3a individually as well as in combination with MCF-7 and MDA-MB-231 cells. To assess any potential synergistic effects between auranofin and nutlin-3a, low concentrations of auranofin and nutlin-3a were simultaneously incubated with MCF-7 and MDA-MB-231 cells. Cell viability assay, caspase-3/7 assay, and poly (ADP-ribose) polymerase cleavage revealed that auranofin and nutlin-3a exerted a synergistic effect on cancer cell apoptosis. Isobologram analysis of MCF-7 and MDA-MB-231 cells noted evident synergism between auranofin and nutlin-3a. The combined treatment increased the expression of mitochondrial pro-apoptotic factors such as Bcl-2 associated X protein and Bcl-2 homologous antagonist/killer. Further, combination treatment signi?cantly enhanced reactive oxygen species (ROS) generation in MCF-7 and MDA-MB-231 cells. In conclusion, data demonstrated that combined treatment with auranofin and nutlin-3a exhibited a synergistic action on breast cancer cells and this combination may be considered for use as a novel therapeutic strategy for breast cancer. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Combination treatment with auranofin and nutlin-3a induces synergistic cytotoxicity in breast cancer cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/15287394.2019.1635934 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.82, no.10, pp 626 - 637 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000473859600001 | - |
dc.identifier.scopusid | 2-s2.0-85068221762 | - |
dc.citation.endPage | 637 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 626 | - |
dc.citation.title | JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | - |
dc.citation.volume | 82 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Auranofin | - |
dc.subject.keywordAuthor | nutlin-3a | - |
dc.subject.keywordAuthor | synergistic effect | - |
dc.subject.keywordAuthor | combination treatment | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR P53 | - |
dc.subject.keywordPlus | REACTIVE OXYGEN | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | MUTANT P53 | - |
dc.subject.keywordPlus | P73 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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