외상성 뇌손상 쥐에서 인체 골수유래 줄기세포의 손상부위로의 이동Human Mesenchymal Stem Cells Derived from Bone Marrow in Traumatic Brain Injury of Rat Migrate to the Site of Injury
- Authors
- 강시현; 전민호; 김상태; 조희진
- Issue Date
- 2009
- Publisher
- 대한재활의학회
- Keywords
- Stem cell transplantation; Traumatic brain injury; Neurobehavioral manifestation; Magnetic resonance spectroscopy; Rats
- Citation
- Annals of Rehabilitation Medicine, v.33, no.5, pp 520 - 526
- Pages
- 7
- Journal Title
- Annals of Rehabilitation Medicine
- Volume
- 33
- Number
- 5
- Start Page
- 520
- End Page
- 526
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33361
- ISSN
- 2234-0645
2234-0653
- Abstract
- Objective: To define the migration and differentiation of adult human mesenchymal stem cells (hMSCs) derived from bone marrow, and their effect on neurobehavioral and cognitive improvements, after traumatic brain injury (TBI) in rats.
Method: Two days after TBI, 1×106 hMSCs were injected into the corpus callosum of fifteen rats, on the contralateral side of TBI. Eleven rats received sham-operation as a control group. Neurobehavioral and Barnes maze tests, and magnetic resonance spectroscopy (MRS) were performed on days 1 and 28 after TBI. Immunohistochemical staining was performed to evaluate distribution and differentiation of hMSCs on day 56.
Results: After 28 days, scores on the neurobehavioral test, Barnes maze test, and magnetic resonance spectroscopy (MRS) were higher than on day 1 in both the stem-cell and control groups, but there were no between-group differences. On day 56, injected hMSCs stained positively with MAB- 1281 were distributed in ipsilateral corpus callosum, lesion boundary zone, parietal cortex, and thalamic area around the lateral ventricle.
Conclusion: hMSCs injected to the contralateral side of TBI survive and migrate to various areas of the ipsilateral hemisphere. We observed no neurobehavioral or cognitive improvements in test animals, indicating the need to adjust experimental methodologies including the development of appropriate tests to evaluate neurobehavioral and cognitive functions of rats.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33361)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.