3T3-L1 세포에서 지방세포형성 유도조절자 및 억제조절자의 발현에 대한 platycodin D의 효과
DC Field | Value | Language |
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dc.contributor.author | 이해용 | - |
dc.contributor.author | 강련화 | - |
dc.contributor.author | 조수현 | - |
dc.contributor.author | 김성수 | - |
dc.contributor.author | 김영식 | - |
dc.contributor.author | 윤유식 | - |
dc.date.available | 2019-08-14T06:57:41Z | - |
dc.date.issued | 2009-12 | - |
dc.identifier.issn | 1225-9918 | - |
dc.identifier.issn | 2287-3406 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33622 | - |
dc.description.abstract | Platycodin D, a major component of Platycodi radix, is known to have various activities including anti-inflammatory, anti-hyperlipidemic, anti-tumor activities and others. Recently, it was reported that platycodin D inhibits fat accumulation and adipogenesis. The aim of this study was to investigate whether various adipogenic regulators are modulated by platycodin D treatment during the adipogenesis of 3T3-L1 cells. mRNA levels of terminal markers of adipogenesis such as ADIPOQ (adiponectin) and GLUT (glucose transporter) 4, which were quantified by real time PCR, were decreased by platycodin D treatment. mRNA expression of PPAR (peroxisome proliferator-activated receptor) γ and C/EBP (CCAAT/enhaner binding protein) α, which are central transcription factors of adipogenesis, were also decreased by platycodin D treatment. To elucidate the detailed molecular mechanism of platycodin D-induced inhibition of adipogenesis, we analyzed mRNA expression of upstream regulators of PPARγ and C/EPBα. mRNA levels of the pro-adipogenic regulators, KROX20 and KLF (Kruppel-like factor) 15 were markedly down-regulated by platycodin D treatment. On the other hand, mRNA expression of CHOP (C/EBP homologous protein), an anti-adipogenic regulator, was significantly up-regulated by platycodin D treatment. mRNA levels of other pro-adipogenic regulators, C/EBPβ and C/EPBδ, were not affected by platycodin D treatment, and another anti-adipogenic regulator, C/EBPγ was also not affected by platycodin D treatment. Taken together, these results suggest that platycodin D-induced inhibition of adipogenesis is mediated by gene interactions including the down-regulation of pro-adipogenic regulators, KROX20 and KLF15, and the up-regulation of an anti-adipogenic regulator, CHOP. | - |
dc.format.extent | 7 | - |
dc.publisher | 한국생명과학회 | - |
dc.title | 3T3-L1 세포에서 지방세포형성 유도조절자 및 억제조절자의 발현에 대한 platycodin D의 효과 | - |
dc.title.alternative | Effects of Platycodin D on Gene Expressions of Pro-adipogenic and Anti-adipogenic Regulators in 3T3-L1 Cells | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 생명과학회지, v.19, no.12, pp 1801 - 1807 | - |
dc.identifier.kciid | ART001407206 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 1807 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1801 | - |
dc.citation.title | 생명과학회지 | - |
dc.citation.volume | 19 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Adipogenesis | - |
dc.subject.keywordAuthor | platycodin D | - |
dc.subject.keywordAuthor | 3T3-L1 | - |
dc.subject.keywordAuthor | KROX20 | - |
dc.subject.keywordAuthor | KLF15 | - |
dc.subject.keywordAuthor | CHOP | - |
dc.description.journalRegisteredClass | kci | - |
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