Deoxypodophyllotoxin Induces a Th1 Response and Enhances the Antitumor Efficacy of a Dendritic Cell-based Vaccine
DC Field | Value | Language |
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dc.contributor.author | Lee, Jun Sik | - |
dc.contributor.author | Kim, Dae Hyun | - |
dc.contributor.author | Lee, Chang-Min | - |
dc.contributor.author | Ha, Tae Kwun | - |
dc.contributor.author | Noh, Kyung Tae | - |
dc.contributor.author | Park, Jin Wook | - |
dc.contributor.author | Heo, Deok Rim | - |
dc.contributor.author | Son, Kwang Hee | - |
dc.contributor.author | Jung, In Duk | - |
dc.contributor.author | Lee, Eun Kyung | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.contributor.author | Ahn, Soon-Cheol | - |
dc.contributor.author | Park, Yeong-Min | - |
dc.date.available | 2019-08-16T04:56:15Z | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 1598-2629 | - |
dc.identifier.issn | 2092-6685 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33863 | - |
dc.description.abstract | Background: Dendritic cell (DC)-based vaccines are currently being evaluated as a novel strategy for tumor vaccination and immunotherapy. However, inducing long-term regression in established tumor-implanted mice is difficult. Here, we show that deoxypohophyllotoxin (DPT) induces maturation and activation of bone marrow-derived DCs via Toll-like receptor (TLR) 4 activation of MAPK and NF-κB. Methods: The phenotypic and functional maturation of DPT-treated DCs was assessed by flow cytometric analysis and cytokine production, respectively. DPT-treated DCs was also used for mixed leukocyte reaction to evaluate T cell-priming capacity and for tumor regression against melanoma. Results: DPT promoted the activation of CD8+ T cells and the Th1 immune response by inducing IL-12 production in DCs. In a B16F10 melanoma-implanted mouse model, we demonstrated that DPT-treated DCs (DPT-DCs) enhance immune priming and regression of an established tumor in vivo. Furthermore, migration of DPT-DCs to the draining lymph nodes was induced via CCR7 upregulation. Mice that received DPT-DCs displayed enhanced antitumor therapeutic efficacy, which was associated with increased IFN-γproduction and induction of cytotoxic T lymphocyte activity. Conclusion: These findings strongly suggest that the adjuvant effect of DPT in DC vaccination is associated with the polarization of T effector cells toward a Th1 phenotype and provides a potential therapeutic antitumor immunity. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한면역학회 | - |
dc.title | Deoxypodophyllotoxin Induces a Th1 Response and Enhances the Antitumor Efficacy of a Dendritic Cell-based Vaccine | - |
dc.type | Article | - |
dc.identifier.doi | 10.4110/in.2011.11.1.79 | - |
dc.identifier.bibliographicCitation | Immune Network, v.11, no.1, pp 79 - 94 | - |
dc.identifier.kciid | ART001534581 | - |
dc.description.isOpenAccess | Y | - |
dc.citation.endPage | 94 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 79 | - |
dc.citation.title | Immune Network | - |
dc.citation.volume | 11 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Dendritic cells | - |
dc.subject.keywordAuthor | Deoxypodophyllotoxin | - |
dc.subject.keywordAuthor | Interleukin-12 | - |
dc.subject.keywordAuthor | CTL activity | - |
dc.subject.keywordAuthor | DC-based vaccination | - |
dc.description.journalRegisteredClass | kci | - |
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