Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection
DC Field | Value | Language |
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dc.contributor.author | Lee, Taek | - |
dc.contributor.author | Kim, Ga Hyeon | - |
dc.contributor.author | Kim, Soo Min | - |
dc.contributor.author | Hong, Keonyoung | - |
dc.contributor.author | Kim, Younghun | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Sohn, Hiesang | - |
dc.contributor.author | Min, Junhong | - |
dc.date.available | 2019-08-19T00:55:49Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.issn | 1873-4367 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/34084 | - |
dc.description.abstract | In the present study, we fabricated a label-free avian influenza (AIV H5N1) detection biosensor composed of a multi-functional DNA 3 way-Junction (3 W J) on a hollow Au spike-like nanoparticle (hAuSN) using a localized surface plasmon resonance (LSPR) method. To construct the multi-functional DNA (MF-DNA) as a bioprobe, the 3 W J was introduced. The proposed AIV detection bioprobe should contain three functionalities: target recognition, signal amplification, and connection to substrate. To achieve this goal, each piece of the DNA 3 W J was tailored to a hemagglutinin (HA) binding aptamer, FAM dye and thiol group, respectively. The assembly of each DNA 3 W J functional fragment was then confirmed by TBM-Native PAGE. Moreover, the hAuSN was immobilized on the indium-tin-oxide (ITO) substrate for LSPR measurement. The DNA 3 W J was immobilized onto the hAuSN electrode through the thiol-group of DNA 3 W J. The fabricated DNA 3 W J/hAuSN heterolayer on the ITO substrate was investigated by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). LSPR experiments were conducted to confirm HA protein binding to the DNA 3 W J/ hAuSN -modified electrode. The proposed biosensor can detected the HA protein in PBS buffer (LOD: 1 pM) as well as in the diluted chicken serum (LOD: 1 pM). The present study details a label-free, simple fabrication method consisted of DNA 3 W J/ hAuSN heterolayer that uses easy-to-tailor elements to detect not only AIV but also various viruses detection platform easily. © 2019 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.colsurfb.2019.06.070 | - |
dc.identifier.bibliographicCitation | Colloids and Surfaces B: Biointerfaces, v.182 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000489190200004 | - |
dc.identifier.scopusid | 2-s2.0-85068383518 | - |
dc.citation.title | Colloids and Surfaces B: Biointerfaces | - |
dc.citation.volume | 182 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | AI virus detection | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | HA protein | - |
dc.subject.keywordAuthor | Hollow Au spike-like nanoparticles | - |
dc.subject.keywordAuthor | Localized surface plasmon resonance | - |
dc.subject.keywordAuthor | Multi-functional DNA | - |
dc.subject.keywordPlus | Atomic force microscopy | - |
dc.subject.keywordPlus | Biochemistry | - |
dc.subject.keywordPlus | Biosensors | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | Electrodes | - |
dc.subject.keywordPlus | Enamels | - |
dc.subject.keywordPlus | Field emission microscopes | - |
dc.subject.keywordPlus | Gold | - |
dc.subject.keywordPlus | Nanoparticles | - |
dc.subject.keywordPlus | Plasmons | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Scanning electron microscopy | - |
dc.subject.keywordPlus | Tin oxides | - |
dc.subject.keywordPlus | Viruses | - |
dc.subject.keywordPlus | Avian influenza virus | - |
dc.subject.keywordPlus | Field emission scanning electron microscopy | - |
dc.subject.keywordPlus | Indium tin oxide substrates | - |
dc.subject.keywordPlus | Localized surface plasmon resonance | - |
dc.subject.keywordPlus | Modified electrodes | - |
dc.subject.keywordPlus | Multi-functional | - |
dc.subject.keywordPlus | Signal amplifications | - |
dc.subject.keywordPlus | Virus detection | - |
dc.subject.keywordPlus | Surface plasmon resonance | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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