Electrochemical peptide sensor for diagnosing adenoma-carcinoma transition in colon cancer
DC Field | Value | Language |
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dc.contributor.author | Lim, Jong Min | - |
dc.contributor.author | Ryu, Myung Yi | - |
dc.contributor.author | Yun, Jong Won | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Park, Jong Pil | - |
dc.date.available | 2019-03-08T07:35:57Z | - |
dc.date.issued | 2017-12 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3493 | - |
dc.description.abstract | Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Therefore, more sensitive and early diagnostic methods for CRC are urgently needed. In this study, an efficient electrochemical biosensor for early diagnosis of adenoma-to-carcinoma progression that employs a series of chemically modified affinity peptides was developed. A series of amino acid-substituted and cysteine-incorporated synthetic peptides with flexible linkers was chemically synthesized and immobilized to a gold sensor layer; performance of the sensor was monitored using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides (LRG1 BP1-BP4) specific for the LRG1 biomarker as a target protein were chosen according to a quantitative current decrease and dynamic impedance increase by CV and EIS, respectively. Using EIS, the Kd value of the LRG1 BP3 peptide was found to be 8.3 +/- 2.7 nM. The applicability of the sensor to detect LRG1 proteins was confirmed in human plasma from colorectal adenomas and carcinomas (n = 20 in each group). The detection of LRG1 in accordance with the ARC, value (electron-transfer resistance at the electrode surface) of the sensor layer incorporating LRG1 BP3 peptides showed a statistically significant difference (p < 0.001) between adenomas and carcinomas, indicating that the potential use of this biosensing platform for detecting the CRC biomarker, as well as for monitoring the colorectal adenoma-to-carcinoma transition in an electrochemically miniaturized biosensor (e-chem biosensor) in point-of-care testing, is possible. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Electrochemical peptide sensor for diagnosing adenoma-carcinoma transition in colon cancer | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2017.07.013 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.98, pp 330 - 337 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000407538300046 | - |
dc.identifier.scopusid | 2-s2.0-85021882438 | - |
dc.citation.endPage | 337 | - |
dc.citation.startPage | 330 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 98 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | LRG1 | - |
dc.subject.keywordAuthor | Affinity peptide | - |
dc.subject.keywordAuthor | Sensitivity | - |
dc.subject.keywordAuthor | Electrochemical sensor | - |
dc.subject.keywordPlus | HIGH-AFFINITY PEPTIDES | - |
dc.subject.keywordPlus | PHAGE DISPLAY | - |
dc.subject.keywordPlus | COLORECTAL ADENOMA | - |
dc.subject.keywordPlus | POTENTIAL BIOMARKERS | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | LABEL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CHALLENGES | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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