Dimethyl sulfoxide에 의한 세포내 칼슘이온 농도증가가 안정적으로 형질전환된 초파리 S2 세포에서 재조합 사람 cyclooxygenase 1의 발현에 미치는 영향

Abstract

Dimethyl sulfoxide (DMSO) increased the intracellular calcium ion concentration in stably transfected Drosophila melanogaster S2 cells expressing recombinant cyclooxygenase 1 (COX-1). DMSO did not increase the Drosophila NOS (dNOS) transcript level in calcium chelatortreated cells. Expression of recombinant COX-1 due to DMSO was diminished in cells treated with calcium chelators or channel blockers. Our results indicate that an increased intracellular calcium ion concentration due to DMSO is associated with up-regulation of the dNOS gene, leading to enhanced expression of COX-1.