SPIN90, an adaptor protein, alters the proximity between Rab5 and Gapex5 and facilitates Rab5 activation during EGF endocytosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hwan | - |
dc.contributor.author | Oh, Hyejin | - |
dc.contributor.author | Oh, Young Soo | - |
dc.contributor.author | Bae, Jeomil | - |
dc.contributor.author | Hong, Nan Hyung | - |
dc.contributor.author | Park, Su Jung | - |
dc.contributor.author | Ahn, Suyeon | - |
dc.contributor.author | Lee, Miriam | - |
dc.contributor.author | Rhee, Sangmyung | - |
dc.contributor.author | Lee, Sung Haeng | - |
dc.contributor.author | Jun, Youngsoo | - |
dc.contributor.author | Kim, Sung Hyun | - |
dc.contributor.author | Huh, Yun Hyun | - |
dc.contributor.author | Song, Woo Keun | - |
dc.date.available | 2019-08-30T02:58:18Z | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36415 | - |
dc.description.abstract | During ligand-mediated receptor endocytosis, the small GTPase Rab5 functions in vesicle fusion and trafficking. Rab5 activation is known to require interactions with its guanine nucleotide-exchange factors (GEFs); however, the mechanism regulating Rab5 interactions with GEFs remains unclear. Here, we show that the SH3-adapter protein SPIN90 participates in the activation of Rab5 through the recruitment of both Rab5 and its GEF, Gapex5, to endosomal membranes during epidermal growth factor (EGF)-mediated endocytosis. SPIN90 strongly interacts with the inactive Rab5/GDI2 complex through its C-terminus. In response to EGF signaling, extracellular signal-regulated kinase (ERK)-mediated phosphorylation of SPIN90 at Thr-242 enables SPIN90 to bind Gapex5 through its N-terminal SH3 domain. Gapex5 is a determinant of Rab5 membrane targeting, while SPIN90 mediates the interaction between Gapex5 and Rab5 in a phosphorylation-dependent manner. Collectively, our findings suggest that SPIN90, as an adaptor protein, simultaneously binds inactive Rab5 and Gapex5, thereby altering their spatial proximity and facilitating Rab5 activation. | - |
dc.format.extent | 14 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | SPIN90, an adaptor protein, alters the proximity between Rab5 and Gapex5 and facilitates Rab5 activation during EGF endocytosis | - |
dc.title.alternative | SPIN90, an adaptor protein, alters the proximity between Rab5 and Gapex5 and facilitates Rab5 activation during EGF endocytosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s12276-019-0284-5 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.51, no.7, pp 1 - 14 | - |
dc.identifier.kciid | ART002489750 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000479315900001 | - |
dc.identifier.scopusid | 2-s2.0-85069918319 | - |
dc.citation.endPage | 14 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 51 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | GDP DISSOCIATION INHIBITOR | - |
dc.subject.keywordPlus | NUCLEOTIDE-EXCHANGE | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | GTPASES | - |
dc.subject.keywordPlus | EEA1 | - |
dc.subject.keywordPlus | DISPLACEMENT | - |
dc.subject.keywordPlus | RECRUITMENT | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | VESICLES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.