Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Hong-Rae | - |
dc.contributor.author | Kong, Yoon-Ju | - |
dc.contributor.author | Hong, Soo-Gil | - |
dc.contributor.author | Kim, Keun Pil | - |
dc.date.available | 2019-09-30T02:06:35Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/36666 | - |
dc.description.abstract | During meiosis, exchange of DNA segments occurs between paired homologous chromosomes in order to produce recombinant chromosomes, helping to increase genetic diversity within a species. This genetic exchange process is tightly controlled by the eukaryotic RecA homologs Rad51 and Dmc1, which are involved in strand exchange of meiotic recombination, with Rad51 participating specifically in mitotic recombination. Meiotic recombination requires an interaction between homologous chromosomes to repair programmed double-strand breaks (DSBs). In this study, we investigated the budding yeast meiosis-specific proteins Hop2 and Sae3, which function in the Dmc1-dependent pathway. This pathway mediates the homology searching and strand invasion processes. Mek1 kinase participates in switching meiotic recombination from sister bias to homolog bias after DSB formation. In the absence of Hop2 and Sae3, DSBs were produced normally, but showed defects in the DSB-to-single-end invasion transition mediated by Dmc1 and auxiliary factors, and mutant strains failed to complete proper chromosome segregation. However, in the absence of Mek1 kinase activity, Rad51-dependent recombination progressed via sister bias in the hop2 Delta or sae3 Delta mutants, even in the presence of Dmc1. Thus, Hop2 and Sae3 actively modulate Dmc1-dependent recombination, effectively progressing homolog bias, a process requiring Mek1 kinase activation. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.14348/molcells.2016.0069 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.39, no.7, pp 550 - 556 | - |
dc.identifier.kciid | ART002128883 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000382217500006 | - |
dc.identifier.scopusid | 2-s2.0-85015132063 | - |
dc.citation.endPage | 556 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 550 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 39 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Budding yeast | - |
dc.subject.keywordAuthor | Double-strand breaks | - |
dc.subject.keywordAuthor | Hop2 | - |
dc.subject.keywordAuthor | Recombination | - |
dc.subject.keywordAuthor | Sae | - |
dc.subject.keywordPlus | MEIOTIC RECOMBINATION | - |
dc.subject.keywordPlus | BUDDING YEAST | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | PROTEIN COMPLEX | - |
dc.subject.keywordPlus | DMC1 | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | RESECTION | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | RAD51 | - |
dc.subject.keywordPlus | EXO1 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.