Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis
DC Field | Value | Language |
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dc.contributor.author | Lee, SangWook | - |
dc.contributor.author | Kim, Byung Woo | - |
dc.contributor.author | Shin, Hye-Su | - |
dc.contributor.author | Go, Anna | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.contributor.author | Lee, Dong-Ki | - |
dc.contributor.author | Kim, Soyoun | - |
dc.contributor.author | Jeong, Ok Chan | - |
dc.date.available | 2020-02-10T06:40:54Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 2072-666X | - |
dc.identifier.issn | 2072-666X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37432 | - |
dc.description.abstract | Here, we report a simple and effective method for capturing and displacement of gram-negative bacteria using aptamer-modified microbeads and acoustophoresis. As acoustophoresis allows for simultaneous washing and size-dependent separation in continuous flow mode, we efficiently obtained gram-negative bacteria that showed high affinity without any additional washing steps. The proposed device has a simple and efficient channel design, utilizing a long, square-shaped microchannel that shows excellent separation performance in terms of the purity, recovery, and concentration factor. Microbeads (10 mu m) coated with the GN6 aptamer can specifically bind gram-negative bacteria. After incubation of bacteria culture sample with aptamer affinity bead, gram-negative bacteria-bound microbeads, and other unbound/contaminants can be separated by size with high purity and recovery. The device demonstrated excellent separation performance, with high recovery (up to 98%), high purity (up to 99%), and a high-volume rate (500 mu L/min). The acoustophoretic separation performances were conducted using 5 Gram-negative bacteria and 5 Gram-positive bacteria. Thanks to GN6 aptamer's binding affinity, aptamer affinity bead also showed binding affinity to multiple strains of gram-negative bacteria, but not to gram-positive bacteria. GN6 coated bead can capture Gram-negative bacteria but not Gram-positive bacteria. This study may present a different perspective in the field of early diagnosis in bacterial infectious diseases. In addition to detecting living bacteria or bacteria-derived biomarkers, this protocol can be extended to monitoring the contamination of water resources and may aid quick responses to bioterrorism and pathogenic bacterial infections. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/mi10110770 | - |
dc.identifier.bibliographicCitation | Micromachines, v.10, no.11 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000502255300056 | - |
dc.identifier.scopusid | 2-s2.0-85075551053 | - |
dc.citation.number | 11 | - |
dc.citation.title | Micromachines | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | aptamer | - |
dc.subject.keywordAuthor | acoustophoresis | - |
dc.subject.keywordAuthor | microfluidics | - |
dc.subject.keywordAuthor | gram-negative bacteria | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | MICROFLUIDICS | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | SELECTION | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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