Inhibitory effect of α-amyrin acetate isolated from Fraxinus rhynchophylla on Th17 polarization
DC Field | Value | Language |
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dc.contributor.author | Seo, D.-G. | - |
dc.contributor.author | Kim, S. | - |
dc.contributor.author | Lee, D.K. | - |
dc.contributor.author | Kim, N.Y. | - |
dc.contributor.author | Lee, J.-S. | - |
dc.contributor.author | Hwang, K.W. | - |
dc.contributor.author | Park, S.-Y. | - |
dc.date.available | 2020-04-03T00:56:21Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.issn | 1618-095X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37882 | - |
dc.description.abstract | Background: T helper 17 (Th17) cells, which are differentiated from CD4+ T cells, drive inflammation, leading to autoimmune diseases such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease. Therefore, inhibiting Th17 polarization could be a therapeutic target for inflammatory diseases. Purpose: We investigated the inhibitory effect of Fraxinus rhynchophylla (Oleaceae) on Th17 differentiation and found its active component. Study design: The activity of F. rhynchophylla and its active constituent was verified using CD4+ cells extracted from C57BL/6 mice. Methods: Micro-environment for Th17 polarization was provided to CD4+ cells and the effect of treatment with samples was measured by enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and Western blot. Results: The extract of F. rhynchophylla Hance and its chemical constituent, α-amyrin acetate, which was isolated via bioassay-guided isolation, significantly inhibited Th17 polarization as revealed when interleukin (IL)-17, a characteristic cytokine produced by Th17 cells, was measured. Furthermore, the inhibitory effect of α-amyrin acetate was compared to the amyrin derivatives, α-amyrin and β-amyrin. All displayed a suppressive effect on Th17 polarization and all reduced the expression of single transducer and activator of transcription 3 (STAT3) and retinoic acid receptor-related orphan receptor γt (RORγt), which are crucial transcription factors regulating Th17 differentiation. α-Amyrin acetate, however, exhibited the most prominent effects, which indicates that the functional group, acetate, might strengthen the inhibitory effect on Th17 differentiation. Conclusion: Taken together, these results suggest that the extract of F. rhynchophylla and its active constituent, α-amyrin acetate, could be applied as a potential therapeutic agent for autoimmune disorders such as rheumatoid arthritis. © 2019 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier GmbH | - |
dc.title | Inhibitory effect of α-amyrin acetate isolated from Fraxinus rhynchophylla on Th17 polarization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.phymed.2019.153056 | - |
dc.identifier.bibliographicCitation | Phytomedicine, v.63 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000499699300029 | - |
dc.identifier.scopusid | 2-s2.0-85073706118 | - |
dc.citation.title | Phytomedicine | - |
dc.citation.volume | 63 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Fraxinus | - |
dc.subject.keywordAuthor | Interleukin-17 | - |
dc.subject.keywordAuthor | Th17 | - |
dc.subject.keywordAuthor | α-amyrin acetate | - |
dc.subject.keywordPlus | alpha amyrin | - |
dc.subject.keywordPlus | beta amyrin | - |
dc.subject.keywordPlus | retinoic acid receptor | - |
dc.subject.keywordPlus | STAT3 protein | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | bark | - |
dc.subject.keywordPlus | CD4+ T lymphocyte | - |
dc.subject.keywordPlus | cell polarity | - |
dc.subject.keywordPlus | chemical analysis | - |
dc.subject.keywordPlus | cytokine production | - |
dc.subject.keywordPlus | drug isolation | - |
dc.subject.keywordPlus | drug structure | - |
dc.subject.keywordPlus | enzyme linked immunosorbent assay | - |
dc.subject.keywordPlus | Fraxinus | - |
dc.subject.keywordPlus | Fraxinus rhynchophylla | - |
dc.subject.keywordPlus | lymphocyte differentiation | - |
dc.subject.keywordPlus | microenvironment | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | Th17 cell | - |
dc.subject.keywordPlus | transcription regulation | - |
dc.subject.keywordPlus | Western blotting | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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