Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, J.-H. | - |
dc.contributor.author | Kim, H.-H. | - |
dc.contributor.author | Cho, H.-B. | - |
dc.contributor.author | Cha, Y.J. | - |
dc.contributor.author | Lee, J. | - |
dc.date.available | 2020-04-03T00:56:24Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.issn | 2040-3372 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/37883 | - |
dc.description.abstract | The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity. © 2019 The Royal Society of Chemistry. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/c9nr06160f | - |
dc.identifier.bibliographicCitation | Nanoscale, v.11, no.39, pp 18282 - 18289 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000512634500036 | - |
dc.identifier.scopusid | 2-s2.0-85073124504 | - |
dc.citation.endPage | 18289 | - |
dc.citation.number | 39 | - |
dc.citation.startPage | 18282 | - |
dc.citation.title | Nanoscale | - |
dc.citation.volume | 11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | Amino acids | - |
dc.subject.keywordPlus | Antibodies | - |
dc.subject.keywordPlus | Antigens | - |
dc.subject.keywordPlus | Chemical detection | - |
dc.subject.keywordPlus | Computer viruses | - |
dc.subject.keywordPlus | Diagnosis | - |
dc.subject.keywordPlus | Gold nanoparticles | - |
dc.subject.keywordPlus | Nanoparticles | - |
dc.subject.keywordPlus | Synthesis (chemical) | - |
dc.subject.keywordPlus | Viruses | - |
dc.subject.keywordPlus | Antibody concentration | - |
dc.subject.keywordPlus | Autonomous generation | - |
dc.subject.keywordPlus | Diagnostic performance | - |
dc.subject.keywordPlus | Enzyme linked immunosorbent assay | - |
dc.subject.keywordPlus | Hepatitis A virus | - |
dc.subject.keywordPlus | Quantitative assay | - |
dc.subject.keywordPlus | Quantitative detection | - |
dc.subject.keywordPlus | Quantitative level | - |
dc.subject.keywordPlus | Signal detection | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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