Interaction of Synaptosomal-Associated Protein 25 with Neutral Sphingomyelinase 2: Functional Impact on the Sphingomyelin Pathway
DC Field | Value | Language |
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dc.contributor.author | Won, Jong Hoon | - |
dc.contributor.author | Jeon, Hyung Jun | - |
dc.contributor.author | Kim, Seok Kyun | - |
dc.contributor.author | Shin, In Chul | - |
dc.contributor.author | Jang, Ji Min | - |
dc.contributor.author | Ha, Hae Chan | - |
dc.contributor.author | Back, Moon Jung | - |
dc.contributor.author | Kim, Dae Kyong | - |
dc.date.available | 2020-04-10T02:21:21Z | - |
dc.date.issued | 2020-02-10 | - |
dc.identifier.issn | 0306-4522 | - |
dc.identifier.issn | 1873-7544 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38175 | - |
dc.description.abstract | Neurotransmitter release is mediated by ceramide, which is generated by sphingomyelin hydrolysis. In the present study, we examined whether synaptosomal-associated protein 25 (SNAP-25) is involved in ceramide production and exocytosis. Neutral sphingomyelinase 2 (nSMase2) was partially purified from bovine brain and we found that SNAP-25 was enriched in the nSMase2-containing fractions. In rat synaptosomes and PC12 cells, the immunoprecipitation pellet of anti-SNAP-25 antibody showed higher nSMase activity than the immunoprecipitation pellet of anti-nSMase2 antibody. In PC12 cells, SNAP-25 was colocalized with nSMase2. Transfection of SNAP-25 small interfering RNA (siRNA) significantly inhibited nSMase2 translocation to the plasma membrane. A23187-induced ceramide production was concomitantly reduced in SNAP-25 siRNA-transfected PC12 cells compared with that in scrambled siRNA-transfected cells. Moreover, transfection of SNAP-25 siRNA inhibited dopamine release, whereas addition of C-6-ceramide to the siRNA-treated cells moderately reversed this inhibition. Additionally, nSMase2 inhibition reduced dopamine release. Collectively, our results indicate that SNAP-25 interacts with nSMase2 during ceramide production, which mediates exocytosis and neurotransmitter release. (C) 2019 Published by Elsevier Ltd on behalf of IBRO. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Interaction of Synaptosomal-Associated Protein 25 with Neutral Sphingomyelinase 2: Functional Impact on the Sphingomyelin Pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.neuroscience.2019.08.015 | - |
dc.identifier.bibliographicCitation | NEUROSCIENCE, v.427, pp 1 - 15 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000510019600001 | - |
dc.identifier.scopusid | 2-s2.0-85078753422 | - |
dc.citation.endPage | 15 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | NEUROSCIENCE | - |
dc.citation.volume | 427 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | synaptosomal-associated protein 25 | - |
dc.subject.keywordAuthor | ceramide | - |
dc.subject.keywordAuthor | neutral sphingomyelinase 2 | - |
dc.subject.keywordAuthor | dopamine | - |
dc.subject.keywordAuthor | neurotransmission | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | VESICLE DOCKING | - |
dc.subject.keywordPlus | T-SNARE | - |
dc.subject.keywordPlus | CERAMIDE | - |
dc.subject.keywordPlus | SNAP-25 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | EXOCYTOSIS | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | KINASE | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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