Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Yunjeong | - |
dc.contributor.author | Shin, Jongheok | - |
dc.contributor.author | Yang, Jinkyeong | - |
dc.contributor.author | Kim, Hooyeon | - |
dc.contributor.author | Jung, Younghun | - |
dc.contributor.author | Oh, Hyunseok | - |
dc.contributor.author | Kim, Yougjoon | - |
dc.contributor.author | Hwang, Jaehyeon | - |
dc.contributor.author | Park, Myeongseo | - |
dc.contributor.author | Ban, Choongjin | - |
dc.contributor.author | Jeong, Ki Jun | - |
dc.contributor.author | Kim, Sun-Ki | - |
dc.contributor.author | Kweon, Dae-Hyuk | - |
dc.date.available | 2020-04-10T03:21:01Z | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2296-4185 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38230 | - |
dc.description.abstract | Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form. In this study, we describe a versatile platform for enzyme stabilization inside the cell: Intracellularly Immobilized Enzyme System (IIES). A 1,2-fucosyltransferase from Pedobactor saltans (PsFL) and a 1,3-fucosyltransferase from Helicobacter pylori (HpFL), chosen as model proteins, were fused with Oct-1 DNA-binding domain, which mediated the formation of a plasmid–protein complex. Oct-1 fusion enabled both soluble and stable expression of recombinant proteins in the cytoplasm because the fusion proteins were stabilized on the plasmid like immobilized enzymes bound to solid surface. As a result, Oct-1-fusion proteins exhibited significantly greater product titer and yield than non-fusion proteins. Use of fusion proteins PsFL-Oct-1 with C-terminal Oct-1 and Oct-1-PsFL with N-terminal Oct-1 resulted in ~3- and ~2-fold higher 2′-fucosyllactose titers, respectively, than with the use of PsFL alone. When Oct-1 was fused to HpFL, which requires dimerization through heptad repeats, almost two times more 3-fucosyllactose was produced. Fucosyllactose has been used as a food additive because it has various beneficial effects on human health. We anticipate that IIES using Oct-1 fusion protein developed in this study can be applied to stabilize other unstable enzymes. © Copyright © 2020 Park, Shin, Yang, Kim, Jung, Oh, Kim, Hwang, Park, Ban, Jeong, Kim and Kweon. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Frontiers Media S.A. | - |
dc.title | Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency | - |
dc.type | Article | - |
dc.identifier.doi | 10.3389/fbioe.2019.00444 | - |
dc.identifier.bibliographicCitation | Frontiers in Bioengineering and Biotechnology, v.7 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000509235900001 | - |
dc.identifier.scopusid | 2-s2.0-85078399597 | - |
dc.citation.title | Frontiers in Bioengineering and Biotechnology | - |
dc.citation.volume | 7 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | DNA binding protein | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | intracellularly immobilized enzyme system | - |
dc.subject.keywordAuthor | Oct-1 | - |
dc.subject.keywordAuthor | whole-cell biotransformation | - |
dc.subject.keywordPlus | Bioconversion | - |
dc.subject.keywordPlus | Cells | - |
dc.subject.keywordPlus | Cytology | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | Recombinant proteins | - |
dc.subject.keywordPlus | Stabilization | - |
dc.subject.keywordPlus | Biotransformation process | - |
dc.subject.keywordPlus | DNA-binding protein | - |
dc.subject.keywordPlus | Enzyme stabilization | - |
dc.subject.keywordPlus | Immobilized enzyme | - |
dc.subject.keywordPlus | Oct-1 | - |
dc.subject.keywordPlus | Stabilization of enzymes | - |
dc.subject.keywordPlus | Whole cell biocatalysts | - |
dc.subject.keywordPlus | Whole-cell biotransformations | - |
dc.subject.keywordPlus | Enzymes | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.