Identification and biosynthesis of C-24 ethylidene brassinosteroids in Arabidopsis thaliana
DC Field | Value | Language |
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dc.contributor.author | Roh, Jeehee | - |
dc.contributor.author | Yeom, Hyeon Soong | - |
dc.contributor.author | Jang, Hwanhee | - |
dc.contributor.author | Kim, Sunyoung | - |
dc.contributor.author | Youn, Ji Hyun | - |
dc.contributor.author | Kim, Seong-Ki | - |
dc.date.available | 2019-03-08T07:56:25Z | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 1226-9239 | - |
dc.identifier.issn | 1867-0725 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3841 | - |
dc.description.abstract | Isofucosterol is a major 4-demethylsterol which has an ethylidene group at C-24 in Arabidopsis thaliana. To evaluate the presence of brassinosteroids (BRs) with the same carbon skeleton as that of isofucosterol, a large quantity of A. thaliana was extracted and purified. GC-MS/selected ion monitoring analysis verified that 6-deoxohomodolichosterone and homodolichosterone are present in Arabidopsis. An enzyme solution prepared from wild type Arabidopsis successfully mediated conversion of 6-deoxohomodolichosterone to homodolichosterone. However, a double mutant cyp85a1/cyp85a2 could not catalyze the conversion, implying that in A. thaliana the C-6 oxidation of 6-deoxohomodolichosterone to homodolichosterone seems to be catalyzed by CYP85A1 and/or CYP85A2. In yeast, both heterologously expressed CYP85A1 and CYP85A2 catalyzed the C-6 oxidation of 6- deoxohomodolichosterone to homodolichosterone, but the conversion rate in CYP85A2/V60/WAT21 was significantly higher than that in CYP85A1/V60/WAT21, indicating that C-6 oxidation of 6-deoxohomodolichosterone to homodolichosterone is mainly catalyzed by CYP85A2 in A. thaliana. Taken together, this study strongly suggests that a biosynthetic pathway for the production of 6-deoxohomodolichosterone and homodolichosterone is functional, and CYP85As have important roles in 24-ethylidene biosynthesis in A. thaliana. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Identification and biosynthesis of C-24 ethylidene brassinosteroids in Arabidopsis thaliana | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12374-017-0132-x | - |
dc.identifier.bibliographicCitation | JOURNAL OF PLANT BIOLOGY, v.60, no.5, pp 533 - 538 | - |
dc.identifier.kciid | ART002466165 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000412366700011 | - |
dc.identifier.scopusid | 2-s2.0-85030856246 | - |
dc.citation.endPage | 538 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 533 | - |
dc.citation.title | JOURNAL OF PLANT BIOLOGY | - |
dc.citation.volume | 60 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Arabidopsis thaliana | - |
dc.subject.keywordAuthor | Biosynthesis | - |
dc.subject.keywordAuthor | Brassinosteroids | - |
dc.subject.keywordAuthor | CYP85As | - |
dc.subject.keywordAuthor | Homodolichosterone | - |
dc.subject.keywordPlus | PHASEOLUS-VULGARIS | - |
dc.subject.keywordPlus | CYTOCHROME-P450 | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | CATALYZES | - |
dc.subject.keywordPlus | DOLICHOSTERONE | - |
dc.subject.keywordPlus | HYDROXYLATION | - |
dc.subject.keywordPlus | BRASSINOLIDE | - |
dc.subject.keywordPlus | CASTASTERONE | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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