Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
DC Field | Value | Language |
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dc.contributor.author | Thak, Eun Jung | - |
dc.contributor.author | Yoo, Su Jin | - |
dc.contributor.author | Moon, Hye Yun | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.available | 2020-04-16T09:20:14Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 1567-1356 | - |
dc.identifier.issn | 1567-1364 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38538 | - |
dc.description.abstract | Yeasts are prominent hosts for the production of recombinant proteins from industrial enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways between these unicellular eukaryotic microorganisms and higher eukaryotic organisms has made them a preferential host to produce secretory recombinant proteins. However, there are several bottlenecks, in terms of quality and quantity, restricting their use as secretory recombinant protein production hosts. In this mini-review, we discuss recent developments in synthetic biology approaches to constructing yeast cell factories endowed with enhanced capacities of protein folding and secretion as well as designed targeted post-translational modification process functions. We focus on the new genetic tools for optimizing secretory protein expression, such as codon-optimized synthetic genes, combinatory synthetic signal peptides and copy number-controllable integration systems, and the advanced cellular engineering strategies, including endoplasmic reticulum and protein trafficking pathway engineering, synthetic glycosylation, and cell wall engineering, for improving the quality and yield of secretory recombinant proteins. © 2020 FEMS 2020. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Oxford University Press | - |
dc.title | Yeast synthetic biology for designed cell factories producing secretory recombinant proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/femsyr/foaa009 | - |
dc.identifier.bibliographicCitation | FEMS Yeast Research, v.20, no.2 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000536497400004 | - |
dc.identifier.scopusid | 2-s2.0-85080843974 | - |
dc.citation.number | 2 | - |
dc.citation.title | FEMS Yeast Research | - |
dc.citation.volume | 20 | - |
dc.type.docType | Review | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | protein trafficking pathway engineering | - |
dc.subject.keywordAuthor | secretory recombinant proteins | - |
dc.subject.keywordAuthor | synthetic glycosylation pathway | - |
dc.subject.keywordAuthor | yeast cell factory | - |
dc.subject.keywordPlus | cell protein | - |
dc.subject.keywordPlus | mannose 6 phosphate | - |
dc.subject.keywordPlus | recombinant protein | - |
dc.subject.keywordPlus | secretory protein | - |
dc.subject.keywordPlus | signal peptide | - |
dc.subject.keywordPlus | synthetic DNA | - |
dc.subject.keywordPlus | cell engineering | - |
dc.subject.keywordPlus | computer model | - |
dc.subject.keywordPlus | endoplasmic reticulum | - |
dc.subject.keywordPlus | endoplasmic reticulum associated degradation | - |
dc.subject.keywordPlus | fungal cell wall | - |
dc.subject.keywordPlus | gene dosage | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | glycosylation | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | product recovery | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein glycosylation | - |
dc.subject.keywordPlus | protein secretion | - |
dc.subject.keywordPlus | Review | - |
dc.subject.keywordPlus | synthetic biology | - |
dc.subject.keywordPlus | unfolded protein response | - |
dc.subject.keywordPlus | yeast cell | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Mycology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Mycology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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