Expression and In Vitro Function of Anti-Breast Cancer Llama-Based Single Domain Antibody VHH Expressed in Tobacco Plants
DC Field | Value | Language |
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dc.contributor.author | Park, Se Ra | - |
dc.contributor.author | Lee, Jeong-Hwan | - |
dc.contributor.author | Kim, Kibum | - |
dc.contributor.author | Kim, Taek min | - |
dc.contributor.author | Lee, Seung Ho | - |
dc.contributor.author | Choo, Young-Kug | - |
dc.contributor.author | Kim, Kyung Soo | - |
dc.contributor.author | Ko, Kisung | - |
dc.date.available | 2020-04-16T09:20:17Z | - |
dc.date.issued | 2020-02 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38539 | - |
dc.description.abstract | Overexpression of human epidermal growth factor receptor type 2 (HER2) is considered as a prognostic factor of breast cancer, which is positively associated with recurrence when cancer metastasizes to the lymph nodes. Here, we expressed the single variable domain on a heavy chain (VHH) form of anti-HER2 camelid single domain antibody in tobacco plants and compared its in vitro anticancer activities with the anti-HER2 full size antibody. The gene expression cassette containing anti-HER2 camelid single domain antibody VHH fused to human IgG Fc region with KDEL endoplasmic reticulum (ER) (VHH-FcK) was transferred into the tobacco plant via the Agrobacterium-mediated transformation. The transformants were screened with polymerase chain reaction and Western blot analyses. Enzyme-linked immunosorbent assay (ELISA) confirmed the binding of the purified anti-HER2 VHH-FcK to the HER2-positive breast cancer cell line, SK-BR-3. Migration assay results confirmed anticancer activity of the plant-derived anticancer camelid single chain antibody. Taken together, we confirmed the possibility of using anti-HER2 VHH-FcK as a therapeutic anticancer agent, which can be expressed and assembled and purified from a plant expression system as an alternative antibody production system. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NLM (Medline) | - |
dc.title | Expression and In Vitro Function of Anti-Breast Cancer Llama-Based Single Domain Antibody VHH Expressed in Tobacco Plants | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms21041354 | - |
dc.identifier.bibliographicCitation | International journal of molecular sciences, v.21, no.4 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000522524400181 | - |
dc.identifier.scopusid | 2-s2.0-85079810825 | - |
dc.citation.number | 4 | - |
dc.citation.title | International journal of molecular sciences | - |
dc.citation.volume | 21 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.subject.keywordAuthor | HER2 | - |
dc.subject.keywordAuthor | Herceptin | - |
dc.subject.keywordAuthor | plant antibody | - |
dc.subject.keywordAuthor | VHH antibody | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GLYCANS | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | VACCINES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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