Enhancing neurogenesis of neural stem cells using homogeneous nanohole pattern-modified conductive platform
DC Field | Value | Language |
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dc.contributor.author | Cho, Yeon-Woo | - |
dc.contributor.author | Kim, Da-Seul | - |
dc.contributor.author | Suhito, Intan Rosalina | - |
dc.contributor.author | Han, Dong Keun | - |
dc.contributor.author | Lee, Taek | - |
dc.contributor.author | Kim, Tae-Hyung | - |
dc.date.available | 2020-04-17T03:21:29Z | - |
dc.date.issued | 2020-01-01 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38591 | - |
dc.description.abstract | Biocompatible platforms, wherein cells attach and grow, are important for controlling cytoskeletal dynamics and steering stem cell functions, including differentiation. Among various components, membrane integrins play a key role in focal adhesion of cells (18–20 nm in size) and are, thus, highly sensitive to the nanotopographical features of underlying substrates. Hence, it is necessary to develop a platform/technique that can provide high flexibility in controlling nanostructure sizes. We report a platform modified with homogeneous nanohole patterns, effective in guiding neurogenesis of mouse neural stem cells (mNSCs). Sizes of nanoholes were easily generated and varied using laser interference lithography (LIL), by changing the incident angles of light interference on substrates. Among three different nanohole patterns fabricated on conductive transparent electrodes, 500 nm-sized nanoholes showed the best performance for cell adhesion and spreading, based on F-actin and lamellipodia/filopodia expression. Enhanced biocompatibility and cell adhesion of these nanohole patterns ultimately resulted in the enhanced neurogenesis of mNSCs, based on the mRNAs expression level of the mNSCs marker and several neuronal markers. Therefore, platforms modified with homogeneous nanohole patterns fabricated by LIL are promising for the precise tuning of nanostructures in tissue culture platforms and useful for controlling various differentiation lineages of stem cells. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Enhancing neurogenesis of neural stem cells using homogeneous nanohole pattern-modified conductive platform | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms21010191 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.21, no.1 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000515378000191 | - |
dc.identifier.scopusid | 2-s2.0-85077325008 | - |
dc.citation.number | 1 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 21 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Laser interference lithography | - |
dc.subject.keywordAuthor | Nanotopography | - |
dc.subject.keywordAuthor | Neural stem cell | - |
dc.subject.keywordAuthor | Neuronal differentiation | - |
dc.subject.keywordPlus | beta tubulin | - |
dc.subject.keywordPlus | F actin | - |
dc.subject.keywordPlus | filopodia | - |
dc.subject.keywordPlus | glial fibrillary acidic protein | - |
dc.subject.keywordPlus | microtubule associated protein 2 | - |
dc.subject.keywordPlus | nanomaterial | - |
dc.subject.keywordPlus | transcription factor Sox2 | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | biocompatibility | - |
dc.subject.keywordPlus | cell adhesion | - |
dc.subject.keywordPlus | cell contact | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell function | - |
dc.subject.keywordPlus | cell growth | - |
dc.subject.keywordPlus | cell interaction | - |
dc.subject.keywordPlus | cell migration | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | cell spreading | - |
dc.subject.keywordPlus | cell viability assay | - |
dc.subject.keywordPlus | contact angle | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | field emission scanning electron microscopy | - |
dc.subject.keywordPlus | focal adhesion | - |
dc.subject.keywordPlus | immunofluorescence microscopy | - |
dc.subject.keywordPlus | laser interference photolithography | - |
dc.subject.keywordPlus | light scattering | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nanotechnology | - |
dc.subject.keywordPlus | nerve cell culture | - |
dc.subject.keywordPlus | nerve cell differentiation | - |
dc.subject.keywordPlus | nervous system development | - |
dc.subject.keywordPlus | neural stem cell | - |
dc.subject.keywordPlus | neurite outgrowth | - |
dc.subject.keywordPlus | neurosphere formation | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | reverse transcription polymerase chain reaction | - |
dc.subject.keywordPlus | surface property | - |
dc.subject.keywordPlus | three dimensional printing | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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