섬유아세포에서 민들레 추출물 함유제(AF-343)에 의한 제 1형 교원질 발현 증가
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조수묵 | - |
dc.contributor.author | 김재현 | - |
dc.contributor.author | 김종근 | - |
dc.contributor.author | 박기문 | - |
dc.contributor.author | 조호찬 | - |
dc.contributor.author | 김인수 | - |
dc.contributor.author | 김범준 | - |
dc.contributor.author | 천영진 | - |
dc.contributor.author | 조재위 | - |
dc.date.available | 2020-04-17T07:20:15Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0377-9556 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38684 | - |
dc.description.abstract | We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343(0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation. | - |
dc.format.extent | 5 | - |
dc.publisher | 대한약학회 | - |
dc.title | 섬유아세포에서 민들레 추출물 함유제(AF-343)에 의한 제 1형 교원질 발현 증가 | - |
dc.title.alternative | Increased Expression of Type I Collagen in AF-343 Treated Human Skin Fibroblasts | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 약 학 회 지, v.56, no.1, pp 9 - 13 | - |
dc.identifier.kciid | ART001641517 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 13 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 9 | - |
dc.citation.title | 약 학 회 지 | - |
dc.citation.volume | 56 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Taraxacum | - |
dc.subject.keywordAuthor | type I collagen | - |
dc.subject.keywordAuthor | MMP-1 | - |
dc.subject.keywordAuthor | Smad2/3 | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.