Anti-photoaging and anti-oxidative activities of natural killer cell conditioned medium following UV-B irradiation of human dermal fibroblasts and a reconstructed skin model
DC Field | Value | Language |
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dc.contributor.author | Lee, Sung-Eun | - |
dc.contributor.author | Kwon, Tae-Rin | - |
dc.contributor.author | Kim, Jong Hwan | - |
dc.contributor.author | Lee, Byung-Chul | - |
dc.contributor.author | Oh, Chang Taek | - |
dc.contributor.author | Im, Minju | - |
dc.contributor.author | Hwang, Yu Kyeong | - |
dc.contributor.author | Paik, Sang Hoon | - |
dc.contributor.author | Han, Seungryel | - |
dc.contributor.author | Kim, Jeom-Yong | - |
dc.contributor.author | Kim, Beom Joon | - |
dc.date.available | 2020-04-21T06:20:35Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.issn | 1791-244X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38881 | - |
dc.description.abstract | Conditioned media from various sources comprise numerous growth factors and cytokines and are known to promote the regeneration of damaged tissues. Among these, natural killer cell conditioned medium (NK-CdM) has been shown to stimulate collagen synthesis and the migration of fibroblasts during the wound healing process. With a long-term aim of developing a treatment for skin photoaging, the ability of NK-CdM to prevent ultraviolet-B (UV-B) damage was assessed in neonatal human dermal fibroblasts (NHDFs) and an in vitro reconstructed skin model. The factors present in NK-CdM were profiled using an antibody array analysis. Protein and mRNA levels in UV-B exposed NHDFs treated with NK-CdM were measured by western blotting and quantitative reverse transcription-PCR, respectively. The total antioxidant capacity of NK-CdM was determined to assess its ability to suppress reactive oxygen species. The anti-photoaging effect of NK-CdM was also assessed in a 3D reconstituted human full skin model. NK-CdM induced proliferation of UV-B-treated NHDFs, increased procollagen expression, and decreased matrix metalloproteinase (MMP)-1 expression. NK-CdM also exhibited a potent antioxidant activity as measured by the total antioxidant capacity. NK-CdM inhibited UV-B-induced collagen degradation by inactivating MAPK signaling. NK-CdM also elicited potential anti-wrinkle effects by inhibiting the UV-B-induced increase in MMP-1 expression levels in a 3D reconstituted human full skin model. Taken together, the suppression of both UV-B-induced MMP-1 expression and JNK activation by NK-CdM suggests NK-CdM as a possible candidate anti-skin aging agent. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | Anti-photoaging and anti-oxidative activities of natural killer cell conditioned medium following UV-B irradiation of human dermal fibroblasts and a reconstructed skin model | - |
dc.type | Article | - |
dc.identifier.doi | 10.3892/ijmm.2019.4320 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.44, no.5, pp 1641 - 1652 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000490547400005 | - |
dc.identifier.scopusid | 2-s2.0-85073666001 | - |
dc.citation.endPage | 1652 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1641 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 44 | - |
dc.type.docType | Article | - |
dc.publisher.location | 그리이스 | - |
dc.subject.keywordAuthor | natural killer cell | - |
dc.subject.keywordAuthor | conditioned medium | - |
dc.subject.keywordAuthor | ultraviolet | - |
dc.subject.keywordAuthor | photoaging | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | DEFINITION | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | ECM | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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