Enhanced intracellular delivery of bcg cell wall skeleton into bladder cancer cells using liposomes functionalized with folic acid and pep-1 peptide
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon H.Y. | - |
dc.contributor.author | Yang H.M. | - |
dc.contributor.author | Kim C.H. | - |
dc.contributor.author | Goo Y.T. | - |
dc.contributor.author | Hwang G.Y. | - |
dc.contributor.author | Chang I.H. | - |
dc.contributor.author | Whang Y.M. | - |
dc.contributor.author | Choi Y.W. | - |
dc.date.available | 2020-04-22T05:21:22Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38930 | - |
dc.description.abstract | Although bacillus Calmette–Guérin cell wall skeleton (BCG-CWS) might function as a potential substitute for live BCG, its use in the treatment of bladder cancer remains limited owing to issues such as insolubility and micrometer-size following exposure to an aqueous environment. Thus, to develop a novel nanoparticulate system for efficient BCG-CWS delivery, liposomal encapsulation was carried out using a modified emulsification-solvent evaporation method (targets: Size, <200 nm; encapsulation efficiency, ~60%). Further, the liposomal surface was functionalized with specific ligands, folic acid (FA), and Pep-1 peptide (Pep1), as targeting and cell-penetrating moieties, respectively. Functionalized liposomes greatly increased the intracellular uptake of BCGCWS in the bladder cancer cell lines, 5637 and MBT2. The immunoactivity was verified through elevated cytokine production and a THP-1 migration assay. In vivo antitumor efficacy revealed that the BCG-CWS-loaded liposomes effectively inhibited tumor growth in mice bearing MBT2 tumors. Dual ligand-functionalized liposome was also superior to single ligand-functionalized liposomes. Immunohistochemistry supported the enhanced antitumor effect of BCG-CWS, with IL-6 production and CD4 infiltration. Thus, we conclude that FA- and Pep1-modified liposomes encapsulating BCG-CWS might be a good candidate for bladder cancer treatment with high target selectivity. © 2019 by the authors. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Enhanced intracellular delivery of bcg cell wall skeleton into bladder cancer cells using liposomes functionalized with folic acid and pep-1 peptide | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/pharmaceutics11120652 | - |
dc.identifier.bibliographicCitation | Pharmaceutics, v.11, no.12 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000506874300070 | - |
dc.identifier.scopusid | 2-s2.0-85076617880 | - |
dc.citation.number | 12 | - |
dc.citation.title | Pharmaceutics | - |
dc.citation.volume | 11 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Bacillus Calmette–Guérin | - |
dc.subject.keywordAuthor | Bladder cancer | - |
dc.subject.keywordAuthor | Cell wall skeleton | - |
dc.subject.keywordAuthor | Cell-penetrating peptide | - |
dc.subject.keywordAuthor | Folate | - |
dc.subject.keywordAuthor | Ligand | - |
dc.subject.keywordAuthor | Liposome | - |
dc.subject.keywordAuthor | Targeted delivery | - |
dc.subject.keywordPlus | UROTHELIAL CELLS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | IMMUNOTHERAPY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | NANOCARRIERS | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordPlus | CWS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.