Benzyl Isothiocyanate Induces Apoptosis via Reactive Oxygen Species-Initiated Mitochondrial Dysfunction and DR4 and DR5 Death Receptor Activation in Gastric Adenocarcinoma Cells
DC Field | Value | Language |
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dc.contributor.author | Han K.W.W. | - |
dc.contributor.author | Po W.W. | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.contributor.author | Kim, Hyun Jung | - |
dc.date.available | 2020-04-22T05:21:38Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 2218-273X | - |
dc.identifier.issn | 2218-273X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38934 | - |
dc.description.abstract | Benzyl isothiocyanate (BITC) is known to inhibit the metastasis of gastric cancer cells but further studies are needed to confirm its chemotherapeutic potential against gastric cancer. In this study, we observed cell shrinkage and morphological changes in one of the gastric adenocarcinoma cell lines, the AGS cells, after BITC treatment. We performed 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, a cell viability assay, and found that BITC decreased AGS cell viability. Reactive oxygen species (ROS) analyses using 2',7'-dichlorofluorescin diacetate (DCFDA) revealed that BITC-induced cell death involved intracellular ROS production, which resulted in mitochondrial dysfunction. Additionally, cell viability was partially restored when BITC-treated AGS cells were preincubated with glutathione (GSH). Western blotting indicated that BITC regulated the expressions of the mitochondria-mediated apoptosis signaling molecules, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and cytochrome c (Cyt c). In addition, BITC increased death receptor DR5 expression, and activated the cysteine-aspartic proteases (caspases) cascade. Overall, our results showed that BITC triggers apoptosis in AGS cells via the apoptotic pathways involved in ROS-promoted mitochondrial dysfunction and death receptor activation. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NLM (Medline) | - |
dc.title | Benzyl Isothiocyanate Induces Apoptosis via Reactive Oxygen Species-Initiated Mitochondrial Dysfunction and DR4 and DR5 Death Receptor Activation in Gastric Adenocarcinoma Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/biom9120839 | - |
dc.identifier.bibliographicCitation | Biomolecules, v.9, no.12 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000506636800088 | - |
dc.identifier.scopusid | 2-s2.0-85076379799 | - |
dc.citation.number | 12 | - |
dc.citation.title | Biomolecules | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | benzyl isothiocyanate | - |
dc.subject.keywordAuthor | death receptors | - |
dc.subject.keywordAuthor | gastric cancer | - |
dc.subject.keywordAuthor | natural product | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordPlus | CANCER AGS CELLS | - |
dc.subject.keywordPlus | MEDIATED APOPTOSIS | - |
dc.subject.keywordPlus | REDUCED GLUTATHIONE | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | SMALL-MOLECULE | - |
dc.subject.keywordPlus | TRAIL | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordPlus | SENSITIZES | - |
dc.subject.keywordPlus | ROS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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