An effective and rapid method for RNA preparation from non-conventional yeast species
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Dong Wook | - |
dc.contributor.author | Hong, Chang Pyo | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.available | 2020-05-12T11:35:14Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.issn | 1096-0309 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/39379 | - |
dc.description.abstract | The increased use of high-throughput RNA-based analysis has spurred the demand for rapid and simple preparation of high quality RNA. RNA preparation from non-conventional yeasts having diverse cell wall and morphological characteristics is often inefficient using current methods adapted for the model yeast, Saccharomyces cerevisiae. We report a simple RNA preparation method based on glass bead-mediated breakage in a formamide/EDTA solution. High quality RNA is generated within 15 min from various non-conventional yeasts species. The obtained RNA can be directly used for experimentation without further RNA purification and buffer exchange. © 2019 The Authors | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press Inc. | - |
dc.title | An effective and rapid method for RNA preparation from non-conventional yeast species | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ab.2019.113408 | - |
dc.identifier.bibliographicCitation | Analytical Biochemistry, v.586 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000518176500001 | - |
dc.identifier.scopusid | 2-s2.0-85071724523 | - |
dc.citation.title | Analytical Biochemistry | - |
dc.citation.volume | 586 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | RNA extractionNon-saccharomyces yeastsCell wallHyphae | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAEGENE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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