Modulation of ABA Signaling by Altering VxG Phi L Motif of PP2Cs in Oryza sativa
DC Field | Value | Language |
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dc.contributor.author | Han, Seungsu | - |
dc.contributor.author | Min, Myung Ki | - |
dc.contributor.author | Lee, Su-Youn | - |
dc.contributor.author | Lim, Chae Woo | - |
dc.contributor.author | Bhatnagar, Nikita | - |
dc.contributor.author | Lee, Yeongmok | - |
dc.contributor.author | Shin, Donghyuk | - |
dc.contributor.author | Chung, Ka Young | - |
dc.contributor.author | Lee, Sung Chul | - |
dc.contributor.author | Kim, Beom-Gi | - |
dc.contributor.author | Lee, Sangho | - |
dc.date.available | 2019-03-08T07:57:23Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.issn | 1674-2052 | - |
dc.identifier.issn | 1752-9867 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3944 | - |
dc.description.abstract | The abscisic acid (ABA) signaling pathway is regulated by clade A type 2C protein phosphatases (PP2CAs) in plants. In the presence of ABA, PP2Cs release stress/ABA-activated protein kinases by binding to ABA-bound receptors (PYL/RCARs) for activation. Although the wedging tryptophan in PP2Cs is critical in the interaction with PYL/RCARs in Arabidopsis and rice, it remains elusive as to how other interface regions are involved in the interaction. Here, we report the identification of a conserved region on PP2Cs, termed the VxG Phi L motif, which modulates the interaction with PYL/RCARs through its second and fourth residues. The effects of the second and fourth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis. One OsPP2C50 mutant, VFGML ("FM'') mutant, lowered the affinity to OsPYL/RCAR3 by similar to 15-fold in comparison with the wild-type. Comparison of the crystal structures of wild-type OsPP2C50: ABA: OsPYL/RCAR3 with those composed of FM mutant revealed local conformational changes near the VxG Phi L motif, further supported by hydrogen-deuterium exchange mass spectrometry. In rice protoplasts, ABA signaling was altered by mutations in the VxG Phi L motif. Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM showed altered ABA sensitivity. Taken together, the VxG Phi L motif of PP2Cs appears to modulate the affinity of PP2Cs with PYL/RCARs and thus likely to alter the ABA signaling, leading to the differential sensitivity to ABA in planta. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | CELL PRESS | - |
dc.title | Modulation of ABA Signaling by Altering VxG Phi L Motif of PP2Cs in Oryza sativa | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.molp.2017.08.003 | - |
dc.identifier.bibliographicCitation | MOLECULAR PLANT, v.10, no.9, pp 1190 - 1205 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000410379100006 | - |
dc.identifier.scopusid | 2-s2.0-85029164166 | - |
dc.citation.endPage | 1205 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1190 | - |
dc.citation.title | MOLECULAR PLANT | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | ABA receptor | - |
dc.subject.keywordAuthor | type 2C protein phosphatase | - |
dc.subject.keywordAuthor | VxG Phi L motif | - |
dc.subject.keywordAuthor | Oryza sativa | - |
dc.subject.keywordPlus | PROTEIN PHOSPHATASE 2C | - |
dc.subject.keywordPlus | RAY SOLUTION SCATTERING | - |
dc.subject.keywordPlus | EXCHANGE MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | ABSCISIC-ACID | - |
dc.subject.keywordPlus | BIOLOGICAL MACROMOLECULES | - |
dc.subject.keywordPlus | NEGATIVE REGULATOR | - |
dc.subject.keywordPlus | SEED-GERMINATION | - |
dc.subject.keywordPlus | PYL PROTEINS | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | KINASES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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