Genetic alterations responsible for reduced susceptibility to vancomycin in community-associated MRSA strains of ST72
DC Field | Value | Language |
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dc.contributor.author | Baek, Jin Yang | - |
dc.contributor.author | Chung, Doo Ryeon | - |
dc.contributor.author | Ko, Kwan Soo | - |
dc.contributor.author | Kim, So Hyun | - |
dc.contributor.author | Yang, Soo-Jin | - |
dc.contributor.author | Kang, Cheol-In | - |
dc.contributor.author | Peck, Kyong Ran | - |
dc.contributor.author | Song, Jae-Hoon | - |
dc.date.available | 2019-03-08T07:57:42Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.issn | 0305-7453 | - |
dc.identifier.issn | 1460-2091 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3978 | - |
dc.description.abstract | Objectives: We previously reported the first case of vancomycin treatment failure due to development of vancomycin-intermediate resistance in a patient with an MRSA of ST72, a community genotype in Korea. We investigated two isogenic MRSA strains from this patient, who experienced treatment failure with vancomycin and rifampicin. Methods: We tracked the genetic alterations that confer reduced susceptibility to vancomycin on those two isogenic MRSA strains by WGS. Results: Five non-synonymous mutations were identified, including rpoB (H481Y), dprA (G196C), femA (F92C), vraR (E127K) and agrC (E391stop). We further studied the role of a mutation of vraR in reduced susceptibility to vancomycin. Introduction of the mutated vraR (E127K) into a vancomycin-susceptible Staphylococcus aureus strain resulted in an increase in vraSR mRNA expression and vancomycin MIC and development of the heteroVISA phenotype, which was confirmed by the population analysis profile (PAP)/AUC. Electron microscopy showed increased cell wall thickness in the strains with mutated vraR. Conclusions: Based on the genomic data, molecular experiments and PAP and cell wall analyses, we propose that a single mutation of vraR is associated with the reduced susceptibility to vancomycin in MRSA and further treatment failure. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Genetic alterations responsible for reduced susceptibility to vancomycin in community-associated MRSA strains of ST72 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/jac/dkx175 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, v.72, no.9, pp 2454 - 2460 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000408084700008 | - |
dc.identifier.scopusid | 2-s2.0-85044864604 | - |
dc.citation.endPage | 2460 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2454 | - |
dc.citation.title | JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | - |
dc.citation.volume | 72 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | RESISTANT STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | PANTON-VALENTINE LEUKOCIDIN | - |
dc.subject.keywordPlus | CELL-WALL | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | OXACILLIN | - |
dc.subject.keywordPlus | DAPTOMYCIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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