Gold nanoparticle/MXene for multiple and sensitive detection of oncomiRs based on synergetic signal amplification
DC Field | Value | Language |
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dc.contributor.author | Mohammadniaei, Mohsen | - |
dc.contributor.author | Koyappayil, Aneesh | - |
dc.contributor.author | Sun, Yi | - |
dc.contributor.author | Min, Junhong | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.date.available | 2020-07-15T05:23:12Z | - |
dc.date.issued | 2020-07-01 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/41919 | - |
dc.description.abstract | Multiple and sensitive detection of oncomiRs for accurate cancer diagnostics is still a challenge. Here, a synergetic amplification strategy was introduced by combining a MXene-based electrochemical signal amplification and a duplex-specific nuclease (DSN)-based amplification system for rapid, attomolar and concurrent quantification of multiple microRNAs on a single platform in total plasma. Synthesized MXene-Ti3C2Tx modified with 5 nm gold nanoparticles (AuNPs) was tasted on a dual screen-printed gold electrode to host vast numbers of DNA probes identically co-immobilized on dedicated electrodes. Interestingly, presence of MXene provided biofouling resistance and enhanced the electrochemical signals by almost 4 folds of magnitude, attributed to its specious surface area and remarkable charge mobility. The 5 nm AuNPs were perfectly distributed within the whole flaky architect of the MXene to give rise to the electrochemical performance of MXene and provide the thiol-Au bonding feature. This synergetic strategy reduced the DSN-based biosensors' assay time to 80 min, provided multiplexability, antifouling activity, substantial sensitivity and specificity (single mutation recognition). The limit of detection of the proposed biosensor for microRNA-21 and microRNA-141 was respectively 204 aM and 138 aM with a wide linear range from 500 aM to 50 nM. As a proof of concept, this newly-developed strategy was coupled with a 96-well adaptive sensing device to successfully profile three cancer plasma samples based on their altered oncomiR abundances. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Gold nanoparticle/MXene for multiple and sensitive detection of oncomiRs based on synergetic signal amplification | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2020.112208 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.159 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000531078700014 | - |
dc.identifier.scopusid | 2-s2.0-85083635877 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 159 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | MicroRNA | - |
dc.subject.keywordAuthor | Multiple detection | - |
dc.subject.keywordAuthor | Electrochemical | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.subject.keywordAuthor | Duplex specific nuclease | - |
dc.subject.keywordAuthor | MXene | - |
dc.subject.keywordPlus | CHAIN-REACTION | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | MXENES | - |
dc.subject.keywordPlus | SENSOR | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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