Expression, function, and glycosylation of anti-colorectal cancer large single-chain antibody (LSC) in plant
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong Hwan | - |
dc.contributor.author | Park, Se Ra | - |
dc.contributor.author | Phoolcharoen, Waranyoo | - |
dc.contributor.author | Ko, Kisung | - |
dc.date.available | 2020-08-03T06:24:01Z | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 1863-5466 | - |
dc.identifier.issn | 1863-5474 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/42703 | - |
dc.description.abstract | In this study, transgenic tobacco plants were generated to express anti-colorectal cancer large single chain (LSC) antibody CO17-1A (LSC CO) and LSC CO tagged with the endoplasmic reticulum (ER) retention signal KDEL (LSC COK). The LSC antibodies were constructed by linking the C-terminus of variable region (VL) of the light chain (LC) to the N-terminus of the heavy chain (HC) of mAb CO17-1A with a linker peptide. Reverse-transcription PCR (RT) and immunoblot analyses showed that the LSC CO17-1AK expression level was higher than LSC CO17-1A in plant. In glycosylation analysis, oligomannose type glycan form was observed in LSC COK and plant-specific alpha(1,3)-fucose was observed in LSC CO. Binding activity of both LSC CO17-1A and LSC CO17-1AK to human colorectal cancer cell lines SW480 and SW620 were confirmed using indirect cell enzyme-linked immunosorbent assay (ELISA). In indirect cell ELISA, the LSC antibodies had a higher binding activity than full-size mAb CO17-1AK. In surface plasmon resonance (SPR) assay using epidermal cell adhesion molecule (EpCAM) highly expressed on the human colorectal cancer cells, both LSC antibodies showed a similar binding activity to the full-size mAb CO17-1A. These results indicated that LSC antibodies with functional binding activities to human colorectal cancer cells and EpCAM protein were successfully expressed in the transgenic tobacco plant. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Expression, function, and glycosylation of anti-colorectal cancer large single-chain antibody (LSC) in plant | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s11816-020-00610-z | - |
dc.identifier.bibliographicCitation | PLANT BIOTECHNOLOGY REPORTS, v.14, no.3, pp 363 - 371 | - |
dc.identifier.kciid | ART002599551 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000522176700001 | - |
dc.identifier.scopusid | 2-s2.0-85082501036 | - |
dc.citation.endPage | 371 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 363 | - |
dc.citation.title | PLANT BIOTECHNOLOGY REPORTS | - |
dc.citation.volume | 14 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Anti-cancer | - |
dc.subject.keywordAuthor | Large single chain antibody | - |
dc.subject.keywordAuthor | Plant-derived antibody | - |
dc.subject.keywordAuthor | Transgenic plant | - |
dc.subject.keywordPlus | RECOMBINANT | - |
dc.subject.keywordPlus | VACCINES | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scopus | - |
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