Fabrication of Cell Chip to Detect Dopamine Secretion in PC12 Cells Using Electrochemical Method

Abstract

A cell-based chip was fabricated for in vitro label-free monitoring of dopamine secreted from neural cells. PC12 cells were grown on the chip and allowed for cyclic voltammetric measurement to detect dopamine secretion. A quasireversible redox process was successfully observed from the cellular dopamine, and the voltammetric results showed the positive relationship between the cell numbers and peak current. Since chemical dopamine added to the cell did not change the redox potential of PC12 cells, we concluded that redox peaks of cyclic voltammetry (CV) surely came from the cellular dopamine which is released by PC12 cells. In addition, a stimulatory effect of potassium and glucose on dopamine secretion in PC12 cells was investigated by CV measurement, and further investigated by immunofluorescence staining to validate electrochemical findings.