Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wu, Yixuan | - |
dc.contributor.author | Choi, Namhyun | - |
dc.contributor.author | Chen, Hao | - |
dc.contributor.author | Dang, Hajun | - |
dc.contributor.author | Chen, Lingxin | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-18T07:17:20Z | - |
dc.date.available | 2021-06-18T07:17:20Z | - |
dc.date.issued | 2020-02-04 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/44303 | - |
dc.description.abstract | We report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene, but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform and evaluated its performance by sequentially measuring the Raman signals of B. anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limits of detection (LODs) than RT-qPCR under the small cycle number of 20. Particularly, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15, but SERS-PCR enabled DNA detection after only five cycles with an LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1-1000 pM) is wider than that for RTqPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.9b04522 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.92, no.3, pp 2628 - 2634 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000511509400038 | - |
dc.identifier.scopusid | 2-s2.0-85079017548 | - |
dc.citation.endPage | 2634 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 2628 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 92 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | MULTIPLEX DETECTION | - |
dc.subject.keywordPlus | AMPLIFICATION-FREE | - |
dc.subject.keywordPlus | SERS | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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