Functional fusion proteins and prevention of electrode fouling for a sensitive electrochemical immunosensor
DC Field | Value | Language |
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dc.contributor.author | Kim, A-Ram | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Kim, Minseok S. | - |
dc.contributor.author | Kim, In-Ho | - |
dc.contributor.author | Kim, Ki-Suk | - |
dc.contributor.author | Chung, Kwang Hoe | - |
dc.contributor.author | Ko, Sungho | - |
dc.date.available | 2019-03-08T08:56:10Z | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.issn | 1873-4324 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4439 | - |
dc.description.abstract | A highly sensitive electrochemical immunosensor was developed by preventing electrode fouling and using a novel fusion protein of silica binding polypeptides (SBP)-protein G (ProG) created by recombinant DNA technology as a functional crosslinker for rapid and self-oriented immobilization of antibodies onto silica nanoparticles (SiNPs). Antibody immobilization onto the SiNPs by the SBP-ProG could rapidly be achieved without any chemical treatment. The immunosensor was fabricated through bonding of a partially gold-deposited cyclic olefin copolymer (COC) (top substrate) and gold patterned interdigitated array COC electrode (bottomsubstrate). To prevent electrode fouling, human immunoglobulin G (hIgG) was immobilized onto the ceiling inside the microchannel, instead of the bottom electrode. Alkaline phosphatase (AP)-labeled anti-hIgG was allowed to immunoreact with hIgG on the ceiling, followed by addition of an enzyme to generate an oxidative peak current. A three-fold increase in current was observed fromthe immunosensor without any electrode fouling compared with a control with the protein functionalized electrode. Also, the SiNPs facilely coated with AP-anti-hIgG via the SBP-ProG could increase the electrochemical signal up to 20% larger than that of the AP-anti-hIgG alone. Furthermore, this immunosensor was ultrasensitive with a detection limit of 0.68 pg/mL of a biomarker associated with prostate cancer. (C) 2017 Elsevier B. V. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Functional fusion proteins and prevention of electrode fouling for a sensitive electrochemical immunosensor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.aca.2017.02.026 | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, v.967, pp 70 - 77 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000398436000008 | - |
dc.identifier.scopusid | 2-s2.0-85014672806 | - |
dc.citation.endPage | 77 | - |
dc.citation.startPage | 70 | - |
dc.citation.title | ANALYTICA CHIMICA ACTA | - |
dc.citation.volume | 967 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Electrochemical immunosensor | - |
dc.subject.keywordAuthor | Electrode fouling | - |
dc.subject.keywordAuthor | Silica binding polypeptide | - |
dc.subject.keywordAuthor | Protein G | - |
dc.subject.keywordAuthor | Silica nanoparticle | - |
dc.subject.keywordAuthor | Cyclic olefin copolymer | - |
dc.subject.keywordPlus | PROSTATE-SPECIFIC ANTIGEN | - |
dc.subject.keywordPlus | SILICA NANOPARTICLES | - |
dc.subject.keywordPlus | HORSERADISH-PEROXIDASE | - |
dc.subject.keywordPlus | CONFORMATIONAL-CHANGES | - |
dc.subject.keywordPlus | SURFACE CONCENTRATION | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | CARBON NANOTUBES | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | ORIENTATION | - |
dc.subject.keywordPlus | DENDRIMER | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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