Quantitative Serodiagnosis of Scrub Typhus Using Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Platforms
DC Field | Value | Language |
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dc.contributor.author | Lee, See Hi | - |
dc.contributor.author | Hwang, Joonki | - |
dc.contributor.author | Kim, Kihyun | - |
dc.contributor.author | Jeon, Jinhyeok | - |
dc.contributor.author | Lee, Sangyeop | - |
dc.contributor.author | Ko, Juhui | - |
dc.contributor.author | Lee, Jichul | - |
dc.contributor.author | Kang, Minhee | - |
dc.contributor.author | Chung, Doo Ryeon | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-18T07:23:21Z | - |
dc.date.available | 2021-06-18T07:23:21Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/44651 | - |
dc.description.abstract | A surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) technique has been developed for the rapid and accurate diagnosis of scrub typhus. Lateral flow kits for the detection of O. tsutsugamushi IgG (scrub typhus biomarker) were fabricated, and the calibration curve for various standard clinical sera concentrations were obtained by Raman measurements. The clinical sera titer values were determined by fitting the Raman data to the calibration curve. To assess the clinical feasibility of the proposed method, SERS-LFA assays were performed on 40 clinical samples. The results showed good agreement with those of the standard indirect immunofluorescence assay (IFA) method. SERS-LFA has many advantages over IFA including the less sample volume, simpler assay steps, shorter assay time, more systematic quantitative analysis, and longer assay lifetime. As SERS strips can be easily integrated with a miniaturized Raman spectrophotometer, field serodiagnosis is also more feasible. © 2019 American Chemical Society. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Quantitative Serodiagnosis of Scrub Typhus Using Surface-Enhanced Raman Scattering-Based Lateral Flow Assay Platforms | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.9b02363 | - |
dc.identifier.bibliographicCitation | Analytical Chemistry, v.91, no.19, pp 12275 - 12282 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000488993000021 | - |
dc.identifier.scopusid | 2-s2.0-85071728434 | - |
dc.citation.endPage | 12282 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 12275 | - |
dc.citation.title | Analytical Chemistry | - |
dc.citation.volume | 91 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | Calibration | - |
dc.subject.keywordPlus | Curve fitting | - |
dc.subject.keywordPlus | Raman scattering | - |
dc.subject.keywordPlus | Calibration curves | - |
dc.subject.keywordPlus | Clinical feasibility | - |
dc.subject.keywordPlus | Clinical samples | - |
dc.subject.keywordPlus | Indirect immunofluorescence | - |
dc.subject.keywordPlus | Lateral flow assay | - |
dc.subject.keywordPlus | Raman measurements | - |
dc.subject.keywordPlus | Sample volume | - |
dc.subject.keywordPlus | Surface enhanced Raman Scattering (SERS) | - |
dc.subject.keywordPlus | Surface scattering | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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