Development of a gene carrier using a triblock co-polyelectrolyte with poly(ethylene imine)-poly(lactic acid)-poly(ethylene glycol)
- Authors
- Sim, Taehoon; Park, Gayoung; Min, Hyeyoung; Kang, Soowon; Lim, Chaemin; Bae, Sungmin; Lee, Eun Seong; Youn, Yu Seok; Oh, Kyung Taek
- Issue Date
- May-2017
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- Triblock copolymer; polyelectrolyte; gene delivery; small interference RNA; cancer; polyplex
- Citation
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, v.32, no.3, pp 280 - 292
- Pages
- 13
- Journal Title
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
- Volume
- 32
- Number
- 3
- Start Page
- 280
- End Page
- 292
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4508
- DOI
- 10.1177/0883911516671154
- ISSN
- 0883-9115
1530-8030
- Abstract
- The success of gene therapy mainly depends on the carriers for effective gene delivery. A non-viral vector using a cationic block co-polyelectrolyte, PEI-PLA-PEG polyethyleneimine-poly(lactic acid)-poly(ethylene glycol)) was developed as a potential gene carrier. The cationic PEI-PLA-PEG showed less toxicity compared to PEI and formed a gene nanocomplex (termed polyplex) by interaction with plasmid DNA or small interference RNA. The polyplex showed smaller particle size and greater positive zeta potential by increasing the high polymer nitrogen/DNA phosphate ratio. The polyplex with a nitrogen/DNA phosphate ratio of 16 or 32 demonstrated higher gene transfection by fluorescence imaging, flow cytometry measurement, and -galactosidase activity. In particular, the polyplex with therapeutic histone deacetylase small interference RNA at nitrogen/DNA phosphate ratio 16 showed the most favorable properties with definite tumor growth inhibition. The synthetic PEI-PLA-PEG also showed less toxicity and would, therefore, be a great potential gene carrier, particularly given that small interference RNA delivery does not increase the charge density of small interference RNA due to the formation of a stable complex through conjugation with PLA-PEG.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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