Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Battumur, Urantulkhuur | - |
dc.contributor.author | Lee, Manhee | - |
dc.contributor.author | Bae, Gui Sek | - |
dc.contributor.author | Kim, Chang-Hyun | - |
dc.date.accessioned | 2021-06-18T07:40:54Z | - |
dc.date.available | 2021-06-18T07:40:54Z | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 1011-2367 | - |
dc.identifier.issn | 1976-5517 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45130 | - |
dc.description.abstract | Objective: To isolate and identify new methanogens from the rumen of Holstein steers in Korea. Methods: Representative rumen contents were obtained from three ruminally cannulated Holstein steers (793 +/- 8 kg). Pre-reduced media were used for the growth and isolation of methanogens. Optimum growth temperature, pH, and sodium chloride (NaCl) concentration as well as substrate utilization and antibiotic tolerance were investigated to determine the physiological characteristics of the isolated strain. Furthermore, the isolate was microscopically studied for its morphology. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification. Results: One strain designated as KOR-2 was isolated and found to be a non-motile irregular coccus with a diameter of 0.2 to 0.5 mu m. KOR-2 utilized H-2+CO2 and formate but was unable to metabolize acetate, methanol, trimethylamine, 2-propanol, and isobutanol for growth and methane production. The optimum temperature and pH for the growth of KOR-2 were 38 degrees C and 6.8 to 7.0, respectively, while the optimum NaCl concentration essential for KOR-2 growth was 1.0% (w/v). KOR-2 tolerated ampicillin, penicillin G, kanamycin, spectromycin, and tetra-cycline. In contrast, the cell growth was inhibited by chloramphenicol. Phylogenetic analysis of 16S rRNA and mcrA genes revealed the relatedness between KOR-2 and Methanoculleus bourgensis. Conclusion: Based on the physiological and phylogenetic characteristics, KOR-2 was thought to be a new strain within the genus Methanoculleus and named Methanoculleus bourgensis KOR-2. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC | - |
dc.title | Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers | - |
dc.type | Article | - |
dc.identifier.doi | 10.5713/ajas.18.0409 | - |
dc.identifier.bibliographicCitation | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.32, no.2, pp 241 - 248 | - |
dc.identifier.kciid | ART002433618 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000459276500011 | - |
dc.identifier.scopusid | 2-s2.0-85059582363 | - |
dc.citation.endPage | 248 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 241 | - |
dc.citation.title | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | - |
dc.citation.volume | 32 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Methanogen | - |
dc.subject.keywordAuthor | Rumen | - |
dc.subject.keywordAuthor | 16S rRNA | - |
dc.subject.keywordAuthor | mcrA | - |
dc.subject.keywordAuthor | Methanoculleus bourgensis | - |
dc.subject.keywordPlus | 16S RIBOSOMAL-RNA | - |
dc.subject.keywordPlus | SP-NOV. | - |
dc.subject.keywordPlus | METHANOGENIUM-OLENTANGYI | - |
dc.subject.keywordPlus | METHANE | - |
dc.subject.keywordPlus | FISH | - |
dc.subject.keywordPlus | OLDENBURGENSIS | - |
dc.subject.keywordPlus | POPULATIONS | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | COMMUNITY | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Dairy & Animal Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.