Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide-Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes
DC Field | Value | Language |
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dc.contributor.author | Chinnadayyala, Somasekhar R. | - |
dc.contributor.author | Park, Jinsoo | - |
dc.contributor.author | Choi, Yonghyun | - |
dc.contributor.author | Han, Jae-Hee | - |
dc.contributor.author | Yagati, Ajay Kumar | - |
dc.contributor.author | Cho, Sungbo | - |
dc.date.accessioned | 2021-06-18T07:40:55Z | - |
dc.date.available | 2021-06-18T07:40:55Z | - |
dc.date.issued | 2019-01-02 | - |
dc.identifier.issn | 2076-3417 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45132 | - |
dc.description.abstract | The improved binding ability of graphene-nanoparticle composites to proteins or molecules can be utilized to develop new cell-based assays. In this study, we fabricated reduced graphene oxide-gold nanoparticles (rGO-AuNP) electrodeposited onto a transparent indium tin oxide (ITO) electrode and investigated the feasibility of the electrochemical impedance monitoring of cell growth. The electrodeposition of rGO-AuNP on the ITO was optically and electrochemically characterized in comparison to bare, rGO-, and AuNP-deposited electrodes. The cell growth on the rGO-AuNP/ITO electrode was analyzed via electrochemical impedance measurement together with the microscopic observation of HEK293 cells transfected with a green fluorescent protein expression vector. The results showed that rGO-AuNP was biocompatible and induced an increase in cell adherence to the electrode when compared to the bare, AuNP-, or rGO-deposited ITO electrode. At 54 h cultivation, the average and standard deviation of the saturated normalized impedance magnitude of the rGO-AuNP/ITO electrode was 3.44 +/- 0.16, while the value of the bare, AuNP-, and rGO-deposited ITO electrode was 2.48 +/- 0.15, 2.61 +/- 0.18, and 3.01 +/- 0.25, respectively. The higher saturated value of the cell impedance indicates that the impedimetric cell-based assay has a broader measurement range. Thus, the rGO-AuNP/ITO electrode can be utilized for label-free and real-time impedimetric cell-based assays with wider dynamic range. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Electrochemical Impedance Characterization of Cell Growth on Reduced Graphene Oxide-Gold Nanoparticles Electrodeposited on Indium Tin Oxide Electrodes | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/app9020326 | - |
dc.identifier.bibliographicCitation | APPLIED SCIENCES-BASEL, v.9, no.2 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000459436300116 | - |
dc.identifier.scopusid | 2-s2.0-85060110325 | - |
dc.citation.number | 2 | - |
dc.citation.title | APPLIED SCIENCES-BASEL | - |
dc.citation.volume | 9 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | cell-based assay | - |
dc.subject.keywordAuthor | electrochemical impedance spectroscopy | - |
dc.subject.keywordAuthor | electrodeposition | - |
dc.subject.keywordAuthor | gold nanoparticle | - |
dc.subject.keywordAuthor | graphene oxide | - |
dc.subject.keywordPlus | ULTRASENSITIVE DETECTION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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