3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Sang-Woo | - |
dc.contributor.author | Lee, Kang-Woog | - |
dc.contributor.author | Park, Jae-Hyun | - |
dc.contributor.author | Lee, JunHee | - |
dc.contributor.author | Jung, Cho-Rok | - |
dc.contributor.author | Yu, JunJie | - |
dc.contributor.author | Kim, Hwi-Yool | - |
dc.contributor.author | Kim, Dae-Hyun | - |
dc.date.accessioned | 2021-06-18T07:42:34Z | - |
dc.date.available | 2021-06-18T07:42:34Z | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45263 | - |
dc.description.abstract | Tracheal resection has limited applicability. Although various tracheal replacement strategies were performed using artificial prosthesis, synthetic stents and tissue transplantation, the best method in tracheal reconstruction remains to be identified. Recent advances in tissue engineering enabled 3D bioprinting using various biocompatible materials including living cells, thereby making the product clinically applicable. Moreover, clinical interest in mesenchymal stem cell has dramatically increased. Here, rabbit bone marrow-derived mesenchymal stem cells (bMSC) and rabbit respiratory epithelial cells were cultured. The chondrogenic differentiation level of bMSC cultured in regular media (MSC) and that in chondrogenic media (d-MSC) were compared. Dual cell-containing artificial trachea were manufactured using a 3D bioprinting method with epithelial cells and undifferentiated bMSC (MSC group, n = 6) or with epithelial cells and chondrogenic-differentiated bMSC (d-MSC group, n = 6). d-MSC showed a relatively higher level of glycosaminoglycan (GAG) accumulation and chondrogenic marker gene expression than MSC in vitro. Neo-epithelialization and neo-vascularization were observed in all groups in vivo but neo-cartilage formation was only noted in d-MSC. The epithelial cells in the 3D bioprinted artificial trachea were effective in respiratory epithelium regeneration. Chondrogenic-differentiated bMSC had more neo-cartilage formation potential in a short period. Nevertheless, the cartilage formation was observed only in a localized area. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | 3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms19061624 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.6 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000436506600079 | - |
dc.identifier.scopusid | 2-s2.0-85047943048 | - |
dc.citation.number | 6 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | bone marrow-derived mesenchymal stem cell | - |
dc.subject.keywordAuthor | chondrogenic differentiation | - |
dc.subject.keywordAuthor | three-dimensional bioprinting | - |
dc.subject.keywordAuthor | artificial trachea | - |
dc.subject.keywordAuthor | tissue engineering | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | AIRWAY | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | RECONSTRUCTION | - |
dc.subject.keywordPlus | REPLACEMENTS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | SHEEP | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.