Oral vaccination through voluntary consumption of the convict grouper Epinephelus septemfasciatus with yeast producing the capsid protein of red-spotted grouper nervous necrosis virus
- Authors
- Cho, Seo Young; Kim, Hyoung Jin; Lan, Nguyen Thi; Han, Hyun-Ja; Lee, Deok-Chan; Hwang, Jee Youn; Kwon, Mun-Gyeong; Kang, Bo Kyu; Han, Sang Yoon; Moon, Hyoungjoon; Kang, Hyun Ah; Kim, Hong-Jin
- Issue Date
- May-2017
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Nervous necrosis virus; Oral vaccination; Convict grouper; Saccharomyces cerevisiae; Virus challenge; Immune response
- Citation
- VETERINARY MICROBIOLOGY, v.204, pp 159 - 164
- Pages
- 6
- Journal Title
- VETERINARY MICROBIOLOGY
- Volume
- 204
- Start Page
- 159
- End Page
- 164
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4530
- DOI
- 10.1016/j.vetmic.2017.04.022
- ISSN
- 0378-1135
1873-2542
- Abstract
- Nervous necrosis viruses (NNV) cause serious economic losses in marine fish cultivation. The red-spotted grouper NNV (RGNNV) is the most common species of NNV worldwide. There have been many efforts to develop prophylactic NNV vaccines, and various types of vaccine candidate have been suggested. However, most were designed as injectable vaccines, which are not suitable for large-scale vaccination and cause too much stress to the fish. Oral vaccination through voluntary feeding is an ideal way to provide protective immunity to fish. In the present study, recombinant Saccharomyces cerevisiae producing RGNNV capsid protein was used as oral vaccine. The recombinant yeast was prepared in freeze-dried form after disruption. Convict groupers were divided into three groups, control, and oral and parenteral vaccination groups, each consisting of 700 fishes. The control group received no treatment, the parenteral group received one intraperitoneal injection of RGNNV virus-like particles, and the oral vaccination group consumed feed containing the lysed recombinant yeast; voluntary intake was allowed four times at one-week intervals. Both vaccination groups produced serum RGNNV neutralizing antibody titers of > 10(3) (log 2, 9.96), sustained for at least 95 days post-immunization. In addition, in response to challenge with RGNNV both groups suffered significantly reduced mortality and had reduced brain RGNNV titers. These results indicate that recombinant yeast-based oral fish vaccines have great potential for large-scale vaccination.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
- College of Natural Sciences > Department of Life Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.