Induction of apoptosis by Moutan Cortex Radicis in human gastric cancer cells through the activation of caspases and the AMPK signaling pathway
DC Field | Value | Language |
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dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Han, Min-Ho | - |
dc.contributor.author | Park, Shin-Hyung | - |
dc.contributor.author | Hong, Su-Hyun | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.available | 2019-03-08T08:57:12Z | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 0102-695X | - |
dc.identifier.issn | 1981-528X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4541 | - |
dc.description.abstract | Moutan Cortex Radicis, the root bark of Paeonia x suffruticosa Andrews, Paeoniaceae, has been widely used in traditional medicine therapy. Although it has been shown to possess many pharmacological activities, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated. In the present study, we investigated the pro-apoptotic effects of the ethanol extract of Moutan Cortex Radicis in human gastric cancer AGS cells. Moutan Cortex Radicis treatment inhibited the cell viability of AGS cells in a concentration-dependent manner, which was associated with apoptotic cell death. Moutan Cortex Radicis's induction of apoptosis was connected with the upregulation of death receptor 4, death receptor 5, tumor necrosis factor-related apoptosis-inducing ligand, Fas ligand, and Bax, and the downregulation of Bcl-2 and Bid. Moutan Cortex Radicis treatment also induced the loss of mitochondrial membrane potential (Delta psi m), the proteolytic activation of caspases (-3, -8, and -9), and the degradation of poly(ADPribose) polymerase, an activated caspase-3 substrate protein. However, the pre-treatment of a caspase-3 inhibitor significantly attenuated Moutan Cortex Radicis-induced apoptosis and cell viability reduction. In addition, Moutan Cortex Radicis treatment effectively activated the adenosine monophosphate-activated protein kinase signaling pathway; however, a specific inhibitor of AMPK significantly reduced Moutan Cortex Radicis-induced apoptosis. Overall, the results suggest that the apoptotic activity of Moutan Cortex Radicis may be associated with a caspase-dependent cascade through the activation of both extrinsic and intrinsic signaling pathways connected with adenosine monophosphate-activated protein kinase activation, and Moutan Cortex Radicis as an activator of adenosine monophosphate-activated protein kinase could be a prospective application to treat human cancers. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SOC BRASILEIRA FARMACOGNOSIA | - |
dc.title | Induction of apoptosis by Moutan Cortex Radicis in human gastric cancer cells through the activation of caspases and the AMPK signaling pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bjp.2016.11.003 | - |
dc.identifier.bibliographicCitation | REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v.27, no.3, pp 315 - 323 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000404509500007 | - |
dc.identifier.scopusid | 2-s2.0-85021362434 | - |
dc.citation.endPage | 323 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 315 | - |
dc.citation.title | REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | - |
dc.citation.volume | 27 | - |
dc.type.docType | Article | - |
dc.publisher.location | 브라질 | - |
dc.subject.keywordAuthor | Moutan Cortex Radicis | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordPlus | PROTEIN-KINASE AMPK | - |
dc.subject.keywordPlus | PAEONIA-SUFFRUTICOSA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | ETHANOL EXTRACT | - |
dc.subject.keywordPlus | ROOT BARK | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordPlus | METFORMIN | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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