Clinical validation of surface-enhanced Raman scattering-based immunoassays in the early diagnosis of rheumatoid arthritis
DC Field | Value | Language |
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dc.contributor.author | Chon, Hyangah | - |
dc.contributor.author | Wang, Rui | - |
dc.contributor.author | Lee, Sangyeop | - |
dc.contributor.author | Bang, So-Young | - |
dc.contributor.author | Lee, Hye-Soon | - |
dc.contributor.author | Bae, Sang-Cheol | - |
dc.contributor.author | Hong, Sung Hyun | - |
dc.contributor.author | Yoon, Young Ho | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.contributor.author | deMello, Andrew J. | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-18T09:40:56Z | - |
dc.date.available | 2021-06-18T09:40:56Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.issn | 1618-2650 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45725 | - |
dc.description.abstract | We assessed the clinical feasibility of conducting immunoassays based on surface-enhanced Raman scattering (SERS) in the early diagnosis of rheumatoid arthritis (RA). An autoantibody against citrullinated peptide (anti-CCP) was used as a biomarker, magnetic beads conjugated with CCP were used as substrates, and the SERS nanotags were comprised of anti-human IgG-conjugated hollow gold nanospheres (HGNs). We were able to determine the anti-CCP serum levels successfully by observing the distinctive Raman intensities corresponding to the SERS nanotags. At high concentrations of anti-CCP (> 25 U/mL), the results obtained from the SERS assay confirmed those obtained via an ELISA-based assay. Nevertheless, quantitation via our SERS-based assay is significantly more accurate at low concentrations (< 25 U/mL). In this study, we compared the results of an anti-CCP assay of 74 clinical blood samples obtained from the SERS-based assay to that of a commercial ELISA kit. The results of the anti-CCP-positive group (n = 31, > 25 U/mL) revealed a good correlation between the ELISA and SERS-based assays. However, in the anti-CCP-negative group (n = 43, < 25 U/mL), the SERS-based assay was shown to be more reproducible. Accordingly, we suggest that SERS-based assays are novel and potentially useful tools in the early diagnosis of RA. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Clinical validation of surface-enhanced Raman scattering-based immunoassays in the early diagnosis of rheumatoid arthritis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00216-015-9020-8 | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.407, no.27, pp 8353 - 8362 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000362974100021 | - |
dc.identifier.scopusid | 2-s2.0-85027916847 | - |
dc.citation.endPage | 8362 | - |
dc.citation.number | 27 | - |
dc.citation.startPage | 8353 | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 407 | - |
dc.type.docType | Article | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Surface-enhanced Raman scattering | - |
dc.subject.keywordAuthor | Anti-CCP | - |
dc.subject.keywordAuthor | Rheumatoid arthritis | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | Early diagnosis | - |
dc.subject.keywordPlus | CYCLIC CITRULLINATED PEPTIDE | - |
dc.subject.keywordPlus | PATTERNED MICROARRAY CHIP | - |
dc.subject.keywordPlus | SERS-BASED IMMUNOASSAY | - |
dc.subject.keywordPlus | CANCER MARKERS | - |
dc.subject.keywordPlus | IMMUNOGOLD NANOPARTICLES | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | GOLD NANOSPHERES | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | NANOPROBES | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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