3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Dong-Ku | - |
dc.contributor.author | Gong, Xiuqing | - |
dc.contributor.author | Cho, Soongwon | - |
dc.contributor.author | Kim, Jin-young | - |
dc.contributor.author | Edel, Joshua B. | - |
dc.contributor.author | Chang, Soo-Ik | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | deMello, Andrew J. | - |
dc.date.accessioned | 2021-06-18T09:40:58Z | - |
dc.date.available | 2021-06-18T09:40:58Z | - |
dc.date.issued | 2015-11-03 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45727 | - |
dc.description.abstract | Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | 3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.5b02402 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.87, no.21, pp 10770 - 10778 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000364354900020 | - |
dc.identifier.scopusid | 2-s2.0-84946762216 | - |
dc.citation.endPage | 10778 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 10770 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 87 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | SINGLE-CELL ANALYSIS | - |
dc.subject.keywordPlus | PICOLITER-VOLUME | - |
dc.subject.keywordPlus | HODGKINS-DISEASE | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CASPASE-3 | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | GRADIENTS | - |
dc.subject.keywordPlus | MOLECULE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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