Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection
DC Field | Value | Language |
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dc.contributor.author | Chon, H. | - |
dc.contributor.author | Lee, S. | - |
dc.contributor.author | Wang, R. | - |
dc.contributor.author | Bang, S. -Y. | - |
dc.contributor.author | Lee, H. -S. | - |
dc.contributor.author | Bae, S. -C. | - |
dc.contributor.author | Hong, S. H. | - |
dc.contributor.author | Yoon, Y. H. | - |
dc.contributor.author | Lim, D. | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-18T09:41:30Z | - |
dc.date.available | 2021-06-18T09:41:30Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0277-786X | - |
dc.identifier.issn | 1996-756X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/45770 | - |
dc.description.abstract | We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa(TM) CCP and BioPlex(TM) 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0-25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPIE-INT SOC OPTICAL ENGINEERING | - |
dc.title | Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection | - |
dc.type | Article | - |
dc.identifier.doi | 10.1117/12.2189760 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL CONFERENCE ON NANO-BIO SENSING, IMAGING, AND SPECTROSCOPY 2015, v.9523 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000359600900018 | - |
dc.identifier.scopusid | 2-s2.0-84938718321 | - |
dc.citation.title | INTERNATIONAL CONFERENCE ON NANO-BIO SENSING, IMAGING, AND SPECTROSCOPY 2015 | - |
dc.citation.volume | 9523 | - |
dc.type.docType | Proceedings Paper | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Surface-enhanced Raman scattering | - |
dc.subject.keywordAuthor | anti-cyclic citrullinated peptide antibody | - |
dc.subject.keywordAuthor | rheumatoid arthritis | - |
dc.subject.keywordAuthor | immunoassay | - |
dc.subject.keywordAuthor | hollow gold nanospheres | - |
dc.subject.keywordAuthor | magnetic bead | - |
dc.subject.keywordAuthor | early diagnosis | - |
dc.subject.keywordAuthor | blood sample | - |
dc.subject.keywordPlus | EARLY-DIAGNOSIS | - |
dc.subject.keywordPlus | NANOPROBES | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | TAGS | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scopus | - |
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